Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins

被引：160

作者：

Gans, Pierre ^{[1
]}

Hamelin, Olivier ^{[2
]}

Sounier, Remy ^{[1
]}

Ayala, Isabel ^{[1
,3
]}

Dura, M. Asuncion ^{[1
,3
]}

Amero, Carlos D. ^{[1
,3
]}

Noirclerc-Savoye, Marjolaine ^{[1
]}

Franzetti, Bruno ^{[1
,3
]}

Plevin, Michael J. ^{[1
,3
]}

Boisbouvier, Jerome ^{[1
,3
]}

机构：

[1] UJF, CNRS, CEA, Inst Biol Struct Jean Pierre Ebel, F-38027 Grenoble, France

[2] Lab Chim & Biol Metaux, Grenoble, France

[3] CNRS, F-75700 Paris, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 11期

关键词：

isotopic labeling; methyl-group labeling; NMR spectroscopy; protein assemblies; stereospecific protonation; MALATE-SYNTHASE-G; SUPRAMOLECULAR COMPLEXES; C-13; ASSIGNMENTS; VALINE; N-15; PRECURSOR; RESIDUES; DYNAMICS; STRATEGY;

D O I：

10.1002/anie.200905660

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Sometimes less is more: [13C 1H3]methyl isotopomers can be biosynthetically incorporated specifically into the pro-S methyl groups of leucine and valine residues in large protein assemblies within a perdeuterated background by using an acetolactate precursor. This stereospecific labeling strategy considerably enhances NMR spectra for large protein assemblies. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1958 / 1962

页数：5

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