Matrix metalloproteinases and tissue inhibitors of metalloproteinases in murine B-coronavirus-induced neuroinflammation

被引:6
作者
Sengupta, Sourodip [1 ]
Addya, Sankar [2 ]
Biswas, Diptomit [1 ]
Banerjee, Paromita [1 ]
Sarma, Jayasri Das [1 ]
机构
[1] Indian Inst Sci Educ & Res Kolkata IISER K, Dept Biol Sci, Mohanpur, India
[2] Thomas Jefferson Univ, Sidney Kimmel Canc Ctr, Dept Canc Biol, Philadelphia, PA USA
关键词
beta-coronavirus (beta-CoV); MHV-A59; MMPs; ROS; TIMPs; Neuroinflammation; NF-KAPPA-B; SPIKE PROTEIN; MULTIPLE-SCLEROSIS; VIRUS ENTRY; ACTIVATION; CELL; EXPRESSION; CLEAVAGE; SYSTEM; SARS;
D O I
10.1016/j.virol.2021.11.012
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Mouse hepatitis virus (MHV; m-B-CoV) serves as a useful model for studying the cellular factors involved in neuroinflammation. To understand the role of matrix metalloproteinases (MMPs) in neuroinflammation, brain tissues from m-B-CoV-infected mice were harvested at different days post-infection (d.p.i) and investigated for Mmp expression by RT-qPCR. Mmp-2,-3,-8,-12 showed significant mRNA upregulation peaking with viral replication between 5 and 6 d.p.i. Elevated levels of MMP regulator TIMP-1 are suggestive of a TIMP-1 mediated host antiviral response. Biological network assessment suggested a direct involvement of MMP-3,-8,-14 in facilitating peripheral leukocyte infiltrations. Flow cytometry confirmed the increased presence of NK cells, CD4(+) and CD8(+) T cells, neutrophils, and MHCII expressing cells in the m-B-CoV infected mice brain. Our study revealed that m-B-CoV upregulated Park7, RelA, Nrf2, and Hmox1 transcripts involved in ROS production and antioxidant pathways, describing the possible nexus between oxidative pathways, MMPs, and TIMP in m-B-CoV-induced neuroinflammation.
引用
收藏
页码:122 / 135
页数:14
相关论文
共 74 条
[1]   Co-infection of respiratory bacterium with severe acute respiratory syndrome coronavirus induces an exacerbated pneumonia in mice [J].
Ami, Yasushi ;
Nagata, Noriyo ;
Shirato, Kazuya ;
Watanabe, Rie ;
Iwata, Naoko ;
Nakagaki, Keiko ;
Fukushi, Shuetsu ;
Saijo, Masayuki ;
Morikawa, Shigeru ;
Taguchi, Fumihiro .
MICROBIOLOGY AND IMMUNOLOGY, 2008, 52 (02) :118-127
[2]   Elastase-mediated Activation of the Severe Acute Respiratory Syndrome Coronavirus Spike Protein at Discrete Sites within the S2 Domain [J].
Belouzard, Sandrine ;
Madu, Ikenna ;
Whittaker, Gary R. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (30) :22756-22761
[3]   Pathogenesis of Murine Coronavirus in the Central Nervous System [J].
Bender, Susan J. ;
Weiss, Susan R. .
JOURNAL OF NEUROIMMUNE PHARMACOLOGY, 2010, 5 (03) :336-354
[4]   Coronavirus infection of the central nervous system: host-virus stand-off [J].
Bergmann, CC ;
Lane, TE ;
Stohlman, SA .
NATURE REVIEWS MICROBIOLOGY, 2006, 4 (02) :121-132
[5]   Cleavage and Activation of the Severe Acute Respiratory Syndrome Coronavirus Spike Protein by Human Airway Trypsin-Like Protease [J].
Bertram, Stephanie ;
Glowacka, Ilona ;
Mueller, Marcel A. ;
Lavender, Hayley ;
Gnirss, Kerstin ;
Nehlmeier, Inga ;
Niemeyer, Daniela ;
He, Yuxian ;
Simmons, Graham ;
Drosten, Christian ;
Soilleux, Elizabeth J. ;
Jahn, Olaf ;
Steffen, Imke ;
Poehlmann, Stefan .
JOURNAL OF VIROLOGY, 2011, 85 (24) :13363-13372
[6]   Inhibition of transcription factor NF-κB reduces matrix metalloproteinase-1,-3 and-9 production by vascular smooth muscle cells [J].
Bond, M ;
Chase, AJ ;
Baker, AH ;
Newby, AC .
CARDIOVASCULAR RESEARCH, 2001, 50 (03) :556-565
[7]   Cathepsin L functionally cleaves the severe acute respiratory syndrome coronavirus class I fusion protein upstream of rather than adjacent to the fusion peptide [J].
Bosch, Berend Jan ;
Bartelink, Willem ;
Rottier, Peter J. M. .
JOURNAL OF VIROLOGY, 2008, 82 (17) :8887-8890
[8]   Myd88 Initiates Early Innate Immune Responses and Promotes CD4 T Cells during Coronavirus Encephalomyelitis [J].
Butchi, Niranjan ;
Kapil, Parul ;
Puntambekar, Shweta ;
Stohlman, Stephen A. ;
Hinton, David R. ;
Bergmann, Cornelia C. .
JOURNAL OF VIROLOGY, 2015, 89 (18) :9299-9312
[9]   Intracellular substrate cleavage: a novel dimension in the biochemistry, biology and pathology of matrix metalloproteinases [J].
Cauwe, Benedicte ;
Opdenakker, Ghislain .
CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2010, 45 (05) :351-423
[10]   CD4 Deficiency Causes Poliomyelitis and Axonal Blebbing in Murine Coronavirus-Induced Neuroinflammation [J].
Chakravarty, Debanjana ;
Saadi, Fareeha ;
Kundu, Soumya ;
Bose, Abhishek ;
Khan, Reas ;
Dine, Kimberly ;
Kenyon, Lawrence C. ;
Shindler, Kenneth S. ;
Das Sarma, Jayasri .
JOURNAL OF VIROLOGY, 2020, 94 (14)