Nitroxide radicals protect against DNA damage in rat epithelial cells induced by nitric oxide, nitroxyl anion and peroxynitrite

被引:22
作者
Fedeli, D
Damiani, E
Greci, L
Littarru, GP
Falcioni, G
机构
[1] Univ Camerino, Dipartimento Biol MCA, I-62032 Camerino, MC, Italy
[2] Univ Ancona, Dipartimento Sci Mat & Terra, I-60131 Ancona, Italy
[3] Univ Ancona, Fac Med, Ist Biochim, I-60100 Ancona, Italy
关键词
aromatic and aliphatic nitroxides; nitric oxide; peroxynitrite; nitroxyl anion; DNA damage; comet assay; antioxidants;
D O I
10.1016/S1383-5718(02)00296-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In order to gain more knowledge on the antioxidant role of nitroxide radicals, in this study we investigate their possible protective action against DNA damage induced by nitric oxide (NO) and reactive nitrogen oxide species deriving from it, namely nitroxyl anion (NO-) and peroxynitrite (ONOO-). Rat trachea epithelial cells were exposed under aerobic conditions to (1) NO generated by 150 muM S-nitrosoglutathione monoethyl ester (GSNO-MEE), (2) NO- generated by 200 muM Angeli's salt (Na2N2O3) (3) ONOO- generated by 1 mM SIN-1 (3-morpholino-sydnonimine) and (4) 100 muM synthesized ONOO-, in the absence and presence of 5 muM of two indolinonic nitroxides synthesized by us and the piperidine nitroxide TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl). DNA damage was assessed using the comet assay-a rapid and sensitive, single-cell gel electrophoresis technique used to detect primary DNA damage in individual cells. The parameter tail moment, used as an index of DNA damage, showed that in all cases the nitroxides remarkably inhibited DNA strand breaks induced by the different nitrogen oxide species. All three nitroxides protect to the same extent, except in the case of synthesized peroxynitrite where the aromatic nitroxides 1 and 2 are more efficient than TEMPO. These findings are consistent with the antioxidant character of nitroxide compounds and give additional information on the potential implications for their use as therapeutic agents. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:117 / 125
页数:9
相关论文
共 44 条
[21]   Nitric oxide-induced autophagy and the activation of activated protein kinase pathway protect against apoptosis in human dental pulp cells [J].
Park, S. Y. ;
Park, M. Y. ;
Park, H. G. ;
Lee, K. J. ;
Kook, M. S. ;
Kim, W. J. ;
Jung, J. Y. .
INTERNATIONAL ENDODONTIC JOURNAL, 2017, 50 (03) :260-270
[22]   Reciprocal paracrine interactions between normal human epithelial and mesenchymal cells protect cellular DNA from radiation-induced damage [J].
Nakazawa, Yuka ;
Saenko, Vladimir ;
Rogounovitch, Tatiana ;
Suzuki, Keiji ;
Mitsutake, Norisato ;
Matsuse, Michiko ;
Yamashita, Shunichi .
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 2008, 71 (02) :567-577
[23]   The protective activities of water-soluble C60 derivatives against nitric oxide-induced cytotoxicity in rat pheochromocytoma cells [J].
Hu, Zhen ;
Huang, Yudong ;
Guan, Wenchao ;
Zhang, Jinglong ;
Wang, Feng ;
Zhao, Lei .
BIOMATERIALS, 2010, 31 (34) :8872-8881
[24]   Protective effect of diphlorethohydroxycarmalol against oxidative stress-induced DNA damage and apoptosis in retinal pigment epithelial cells [J].
Park, Cheol ;
Lee, Hyesook ;
Hong, Su Hyun ;
Kim, Jeong-Hwan ;
Park, Seh-Kwang ;
Jeong, Ji-Won ;
Kim, Gi-Young ;
Hyun, Jin Won ;
Yun, Seok Joong ;
Kim, Byung Woo ;
Kim, Wun-Jae ;
Choi, Yung Hyun .
CUTANEOUS AND OCULAR TOXICOLOGY, 2019, 38 (03) :298-308
[25]   Nitric oxide synthase like activity-dependent nitric oxide production protects against chilling-induced oxidative damage in Chorispora bungeana suspension cultured cells [J].
Liu, Yajie ;
Jiang, Haifeng ;
Zhao, Zhiguang ;
An, Lizhe .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2010, 48 (12) :936-944
[26]   Role of nitric oxide in NAG-ST induced store-operated calcium entry in rat intestinal epithelial cells [J].
Hoque, KM ;
Saha, S ;
Gupta, DD ;
Chakrabarti, MK .
TOXICOLOGY, 2004, 201 (1-3) :95-103
[27]   Calcium channel blockers protect against aluminium-induced DNA damage and block adaptive response to genotoxic stress in plant cells [J].
Achary, V. Mohan M. ;
Parinandi, Narasimham L. ;
Panda, Brahma B. .
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2013, 751 (02) :130-138
[28]   Selenised yeast sources differ in their capacity to protect porcine jejunal epithelial cells from cadmium-induced toxicity and oxidised DNA damage [J].
Lynch, Sarah ;
Horgan, Karina ;
Walls, Dermot ;
White, Blanaid .
BIOMETALS, 2018, 31 (05) :845-858
[29]   Selenised yeast sources differ in their capacity to protect porcine jejunal epithelial cells from cadmium-induced toxicity and oxidised DNA damage [J].
Sarah Lynch ;
Karina Horgan ;
Dermot Walls ;
Blánaid White .
BioMetals, 2018, 31 :845-858
[30]   Interaction of nitric oxide and transforming growth factor-β1 induced by angiotensin II and mechanical stretch in rat renal tubular epithelial cells [J].
Miyajima, A ;
Chen, J ;
Kirman, I ;
Poppas, DP ;
Vaughan, ED ;
Felsen, D .
JOURNAL OF UROLOGY, 2000, 164 (05) :1729-1734