Qualitative and quantitative analysis of anthraquinone derivatives in rhizomes of tissue culture-raised Rheum emodi Wall. plants

被引：48

作者：

Malik, Sonia ^{[1
]}

Sharma, Nandini ^{[1
]}

Sharma, Upendra K. ^{[1
]}

Singh, Narendra P. ^{[1
]}

Bhushan, Shashi ^{[1
]}

Sharma, Madhu ^{[1
]}

Sinha, Arun K. ^{[1
]}

Ahuja, Paramvir S. ^{[1
]}

机构：

[1] Inst Himalayan Bioresource Technol, Palampur 176061, HP, India

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2010年 / 167卷 / 09期

关键词：

Anthraquinone; Plant tissue culture; Polygonaceae; Rheum emodi; Secondary metabolite; RAPID CLONAL PROPAGATION; RHUBARB; MICROPROPAGATION; CHROMATOGRAPHY; CONSTITUENTS; CONSERVATION; REGENERATION; GLYCOSIDES; TIPS;

D O I：

10.1016/j.jplph.2009.12.007

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

This paper presents quantification of five anthraquinone derivatives (emodin glycoside, chrysophanol glycoside, emodin, chrysophanol and physcion) in rhizomes of hardened micro-propagated Rheum emodi plants using high-performance liquid chromatography (HPLC). Aseptic shoot cultures were raised using rhizome buds. Shoot multiplication occurred in both agar gelled and liquid Murashige and Skoog (MS) medium supplemented with 10.0 mu M 6-benzylaminopurine (BAP) and 5.0 mu M indole-3-butyric acid (IBA). Rooted plantlets obtained on plant growth regulator (PGR)-free medium were transferred to soil with 92% survival. HPLC analysis revealed the presence of five anthraquinone derivatives: emodin glycoside, chrysophanol glycoside, emodin, chrysophanol and physcion in rhizomes of tissue culture-raised plants. Only emodin glycoside (1) and chrysophanol glycoside (2) were present in 6-month-old hardened tissue cultured plants. In addition, the other three derivatives (emodin (3), chrysophanol (4) and physcion (5)) were also detected after 9 months. (C) 2010 Elsevier GmbH. All rights reserved.

引用

页码：749 / 756

页数：8

共 27 条

[1] Antifungal activity of anthraquinone derivatives from Rheum emodi [J].