Cell-sized mechanosensitive and biosensing compartment programmed with DNA

被引：56

作者：

Majumder, Sagardip ^{[1
]}

Garamella, Jonathan ^{[2
]}

Wang, Ying-Lin ^{[3
]}

DeNies, Maxwell ^{[4
]}

Noireaux, Vincent ^{[2
]}

Liu, Allen P. ^{[1
,4
,5
,6
]}

机构：

[1] Univ Michigan, Dept Mech Engn, Ann Arbor, MI 48109 USA

[2] Univ Minnesota, Dept Phys & Astron, Minneapolis, MN 55455 USA

[3] Natl Cheng Kung Univ, Dept Biomed Engn, Tainan, Taiwan

[4] Univ Michigan, Cellular & Mol Biol Program, Ann Arbor, MI 48109 USA

[5] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA

[6] Univ Michigan, Biophys Program, Ann Arbor, MI 48109 USA

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 53期

关键词：

FREE PROTEIN-SYNTHESIS; ARTIFICIAL CELLS; FREE EXPRESSION; CIRCUITS; BIOLOGY; SYSTEM;

D O I：

10.1039/c7cc03455e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The bottom-up construction of cell-sized compartments programmed with DNA that are capable of sensing the chemical and physical environment remains challenging in synthetic cell engineering. Here, we construct mechanosensitive liposomes with biosensing capability by expressing the E. coli channel MscL and a calcium biosensor using cell-free expression.

引用

页码：7349 / 7352

页数：4

共 27 条

[1] Cell-free protein synthesis: Applications come of age
Carlson, Erik D.
Gan, Rui
Hodgman, C. Eric
Jewett, Michael C.
[J]. BIOTECHNOLOGY ADVANCES, 2012, 30 (05) : 1185 - 1194
[2] Cell-free compartmentalized protein synthesis inside double emulsion templated liposomes with in vitro synthesized and assembled ribosomes
Caschera, Filippo
Lee, Jin Woo
Ho, Kenneth K. Y.
Liu, Allen P.
Jewett, Michael C.
[J]. CHEMICAL COMMUNICATIONS, 2016, 52 (31) : 5467 - 5469
[3] Genetically expanded cell-free protein synthesis using endogenous pyrrolysyl orthogonal translation system
Chemla, Yonatan
Ozer, Eden
Schlesinger, Orr
Noireaux, Vincent
Alfonta, Lital
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 2015, 112 (08) : 1663 - 1672
[4] Removal of the mechanoprotective influence of the cytoskeleton reveals PIEZO1 is gated by bilayer tension
Cox, Charles D.
Bae, Chilman
Ziegler, Lynn
Hartley, Silas
Nikolova-Krstevski, Vesna
Rohde, Paul R.
Ng, Chai-Ann
Sachs, Frederick
Gottlieb, Philip A.
Martinac, Boris
[J]. NATURE COMMUNICATIONS, 2016, 7
[5] Liposome display for in vitro selection and evolution of membrane proteins
Fujii, Satoshi
Matsuura, Tomoaki
Sunami, Takeshi
Nishikawa, Takehiro
Kazuta, Yasuaki
Yomo, Tetsuya
[J]. NATURE PROTOCOLS, 2014, 9 (07) : 1578 - 1591
[6] The All E-coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology
Garamella, Jonathan
Marshall, Ryan
Rustad, Mark
Noireaux, Vincent
[J]. ACS SYNTHETIC BIOLOGY, 2016, 5 (04): : 344 - 355
[7] Giant vesicles functionally expressing membrane receptors for an insect pheromone
Hamada, Satoshi
Tabuchi, Masashi
Toyota, Taro
Sakurai, Takeshi
Hosoi, Tomohiro
Nomoto, Tomonori
Nakatani, Kei
Fujinami, Masanori
Kanzaki, Ryohei
[J]. CHEMICAL COMMUNICATIONS, 2014, 50 (22) : 2958 - 2961
[8] Activation of a Bacterial Mechanosensitive Channel in Mammalian Cells by Cytoskeletal Stress
Heureaux, Johanna
Chen, Di
Murray, Victoria L.
Deng, Cheri X.
Liu, Allen P.
[J]. CELLULAR AND MOLECULAR BIOENGINEERING, 2014, 7 (03) : 307 - 319
[9] Protein aggregation with poly(vinyl) alcohol surfactant reduces double emulsion-encapsulated mammalian cell-free expression
Ho, Kenneth K. Y.
Lee, Jin Woo
Durand, Gregory
Majumder, Sagardip
Liu, Allen P.
[J]. PLOS ONE, 2017, 12 (03):
[10] Mechanically activated artificial cell by using microfluidics
Ho, Kenneth K. Y.
Lee, Lap Man
Liu, Allen P.
[J]. SCIENTIFIC REPORTS, 2016, 6

← 1 2 3 →