Cell-sized mechanosensitive and biosensing compartment programmed with DNA

被引：57

作者：

Majumder, Sagardip ^{[1
]}

Garamella, Jonathan ^{[2
]}

Wang, Ying-Lin ^{[3
]}

DeNies, Maxwell ^{[4
]}

Noireaux, Vincent ^{[2
]}

Liu, Allen P. ^{[1
,4
,5
,6
]}

机构：

[1] Univ Michigan, Dept Mech Engn, Ann Arbor, MI 48109 USA

[2] Univ Minnesota, Dept Phys & Astron, Minneapolis, MN 55455 USA

[3] Natl Cheng Kung Univ, Dept Biomed Engn, Tainan, Taiwan

[4] Univ Michigan, Cellular & Mol Biol Program, Ann Arbor, MI 48109 USA

[5] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA

[6] Univ Michigan, Biophys Program, Ann Arbor, MI 48109 USA

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 53期

关键词：

FREE PROTEIN-SYNTHESIS; ARTIFICIAL CELLS; FREE EXPRESSION; CIRCUITS; BIOLOGY; SYSTEM;

D O I：

10.1039/c7cc03455e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The bottom-up construction of cell-sized compartments programmed with DNA that are capable of sensing the chemical and physical environment remains challenging in synthetic cell engineering. Here, we construct mechanosensitive liposomes with biosensing capability by expressing the E. coli channel MscL and a calcium biosensor using cell-free expression.

引用

页码：7349 / 7352

页数：4

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