Stratum corneum architecture of reconstructed human skin models monitored by fluorescent confocal laser scanning microscopy

Fluorescence confocal scanning laser microscopy (CLSM) using a handheld scanner, was performed to visualize the microscopic architecture of stratum corneum (SC) of the three reconstructed human epidermal (RHE) models: EpiDerm (TM) (MatTek Corporation, Ashland, MA), EPISKIN (R) (EPISKIN SNC, Lyon, France) and SkinEthic (R) (SkinEthic Laboratories, I Nice, France). To compare the differences between the SC structure of the RHE models and human SC, experiments were also performed on normal human epidermis in vivo. Sodium fluorescein stained skin cultures and human skin were imaged continuously using the confocal laser microscope Stratum, Optiscan. Fluorescein was excited at 488 nm and the fluorescent emission was detected at > 505 nm. In each experiment, a series of representative images of each visualized layer of the RHE models and human SC was collected. Our early observations confirmed that the reconstructed human skin models closely resemble human SC. After improving the experimental conditions, the method might be used for studying the effects of topically applied compounds e.g. pharmaceuticals and cosmetic to the SC.