Functional analysis of an archaebacterial voltage-dependent K+ channel

被引:208
作者
Ruta, V [1 ]
Jiang, YX [1 ]
Lee, A [1 ]
Chen, JY [1 ]
MacKinnon, R [1 ]
机构
[1] Rockefeller Univ, Howard Hughes Med Inst, Lab Mol Neurobiol & Biophys, New York, NY 10021 USA
基金
美国国家卫生研究院; 英国惠康基金; 美国国家科学基金会;
关键词
D O I
10.1038/nature01473
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
All living organisms use ion channels to regulate the transport of ions across cellular membranes'. Certain ion channels are classed as voltage-dependent because they have a voltage-sensing structure that induces their pores to open in response to changes in the cell membrane voltage. Until recently, the voltage-dependent K+, Ca2+ and Na+ channels were regarded as a unique development of eukaryotic cells, adapted to accomplish specialized electrical signalling, as exemplified in neurons. Here we present the functional characterization of a voltage-dependent K+ (K-V) channel from a hyperthermophilic archaebacterium from an oceanic thermal vent. This channel possesses all the functional attributes of classical neuronal K-V channels. The conservation of function reflects structural conservation in the voltage sensor as revealed by specific, high-affinity interactions with tarantula venom toxins, which evolved to inhibit eukaryotic K-V channels.
引用
收藏
页码:180 / 185
页数:7
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