共 30 条
Functional analysis of an archaebacterial voltage-dependent K+ channel
被引:208
作者:
Ruta, V
[1
]
Jiang, YX
[1
]
Lee, A
[1
]
Chen, JY
[1
]
MacKinnon, R
[1
]
机构:
[1] Rockefeller Univ, Howard Hughes Med Inst, Lab Mol Neurobiol & Biophys, New York, NY 10021 USA
来源:
基金:
美国国家卫生研究院;
英国惠康基金;
美国国家科学基金会;
关键词:
D O I:
10.1038/nature01473
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
All living organisms use ion channels to regulate the transport of ions across cellular membranes'. Certain ion channels are classed as voltage-dependent because they have a voltage-sensing structure that induces their pores to open in response to changes in the cell membrane voltage. Until recently, the voltage-dependent K+, Ca2+ and Na+ channels were regarded as a unique development of eukaryotic cells, adapted to accomplish specialized electrical signalling, as exemplified in neurons. Here we present the functional characterization of a voltage-dependent K+ (K-V) channel from a hyperthermophilic archaebacterium from an oceanic thermal vent. This channel possesses all the functional attributes of classical neuronal K-V channels. The conservation of function reflects structural conservation in the voltage sensor as revealed by specific, high-affinity interactions with tarantula venom toxins, which evolved to inhibit eukaryotic K-V channels.
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页码:180 / 185
页数:7
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