Ultra fast magic angle spinning solid - state NMR spectroscopy of intact bone

被引：19
作者：
Singh, Chandan [1 ,2 ]
Rai, Ratan Kumar [1 ]
Kayastha, Arvind M. [2 ]
Sinha, Neeraj [1 ]
机构：
[1] Ctr Biomed Res, SGPGIMS Campus,Raebarelly Rd, Lucknow 226014, Uttar Pradesh, India
[2] Banaras Hindu Univ, Fac Sci, Sch Biotechnol, Varanasi 221005, Uttar Pradesh, India
来源：
MAGNETIC RESONANCE IN CHEMISTRY | 2016年 / 54卷 / 02期
关键词：
ssNMR; MAS; H-1; H-1-C-13; NH protons; bone; water; ultra fast magic angle spinning; sensitivity enhancement; NUCLEAR-MAGNETIC-RESONANCE; WATER; MAS; INTERFACE; PROTEIN; RELAXATION; SPECTRA; CALCIUM; FIELD; C-13;
D O I：
10.1002/mrc.4331
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Ultra fastmagic angle spinning (MAS) has been a potent method to significantly average out homogeneous/inhomogeneous line broadening in solid-state nuclear magnetic resonance (ssNMR) spectroscopy. It has given a new direction to ssNMR spectroscopy with its different applications. We present here the first and foremost application of ultra fast MAS (similar to 60 kHz) for ssNMR spectroscopy of intact bone. Thismethodology helps to comprehend and elucidate the organic content in the intact bonematrix with resolution and sensitivity enhancement. At thisMAS speed, amino protons fromorganic part of intact bone start to appear in H-1 NMR spectra. The experimental protocol of ultra-high speed MAS for intact bone has been entailed with an additional insight achieved at 60kHz. Copyright (C) 2015 John Wiley & Sons, Ltd.
引用
收藏
页码：132 / 135
页数：4
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