The truncated AaActin1 promoter is a candidate tool for metabolic engineering of artemisinin biosynthesis in Artemisia annua L.

被引：8

作者：

Li, Yongpeng ^{[1
]}

Chen, Tiantian ^{[1
]}

Liu, Hang ^{[1
]}

Qin, Wei ^{[1
]}

Yan, Xin ^{[1
]}

Wu-Zhang, Kuanyu ^{[1
]}

Peng, Bowen ^{[1
]}

Zhang, Yaojie ^{[1
]}

Yao, Xinghao ^{[1
]}

Fu, Xueqing ^{[1
]}

Li, Ling ^{[1
]}

Tang, Kexuan ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Frontiers Sci Ctr Transformat Mol, Plant Biotechnol Res Ctr, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr,Joi, Shanghai 200240, Peoples R China

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2022年 / 274卷

基金：

中国国家自然科学基金; 比尔及梅琳达.盖茨基金会; 国家重点研发计划;

关键词：

Artemisia annua; AaActin1; promoter; Promoter activity; Artemisinin biosynthesis; 5 ' intron deletion; PARALLEL TRANSCRIPTIONAL REGULATION; ANTIMALARIAL-DRUG; POSITIVE REGULATOR; MOLECULAR-CLONING; GENE-EXPRESSION; UV-B; OVEREXPRESSION; REDUCTASE; SYNTHASE; CYP71AV1;

D O I：

10.1016/j.jplph.2022.153712

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Malaria is a devastating parasitic disease with high levels of morbidity and mortality worldwide. Artemisinin, the active substance against malaria, is a sesquiterpenoid produced by Artemisia annua. To improve artemisinin content in the native A. annua plants, considerable efforts have been attempted, with genetic transformation serving as an effective strategy. Although, the most frequently-used cauliflower mosaic virus (CaMV) 35S (CaMV35S) promoter has proved to be efficient in A. annua transgenic studies, it appears to show weak activity in peltate glandular secretory trichomes (GSTs) of A. annua plants. Here, we characterized the 1727 bp fragment upstream from the translation start codon (ATG) of AaActin1, however, found it was inactive in tobacco. After removal of the 5' intron, the truncated AaActin1 promoter (tpACT) showed 69% and 50% activity of CaMV35S promoter in transiently transformed tobacco and stably transformed A. annua, respectively. beta-glucuronidase (GUS) staining analysis showed that the tpACT promoter was capable of directing the constant expression of a foreign gene in peltate GSTs of transgenic A. annua, representing higher activity than CaMV35S promoter. Collectively, our study provided a novel promoter available for metabolic engineering of artemisinin biosynthesis in A. annua.

引用

页数：9

共 85 条

[1] Lipid Transfer Proteins (AaLTP3 and AaLTP4) Are Involved in Sesquiterpene Lactone Secretion from Glandular Trichomes in Artemisia annua [J].