Citrate attenuates vascular calcification in chronic renal failure rats

被引：11

作者：

Ou, Yan ^{[1
]}

Liu, Zengying ^{[1
]}

Li, Shuiqin ^{[2
]}

Zhu, Xiaojing ^{[1
]}

Lin, Yan ^{[1
]}

Han, Jin ^{[1
]}

Duan, Zhaoyang ^{[1
]}

Jia, Lining ^{[1
]}

Gui, Baosong ^{[1
]}

机构：

[1] Xi An Jiao Tong Univ, Affiliated Hosp 2, Dept Nephrol, 157 Fifth Western St, Xian 710004, Shaanxi, Peoples R China

[2] Yan An Univ, Affiliated Hosp, Dept Rehabil Med, Yanan, Shaanxi, Peoples R China

来源：

APMIS | 2017年 / 125卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Citrate; vascular calcification; renal failure; CHRONIC KIDNEY-DISEASE; BONE; MANAGEMENT; CALCIUM; INJURY; ACID;

D O I：

10.1111/apm.12667

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Vascular calcification (VC) is a major contributor of cardiovascular dysfunction in chronic renal failure (CRF). Citrate binds calcium and inhibits the growth of calcium crystals. This present study intends to evaluate the effect of citrate on VC in adenine-induced CRF rats. The rats were randomly divided into five groups: the control group, the citrate control group, model group, model rats with low-dose treatment of citrate (216 mg/kg) and model rats with high-dose treatment of citrate (746 mg/kg). The rats were euthanized at 5 weeks with their blood and aorta in detection. The results showed that serum level of blood urea nitrogen, serum creatinine, phosphorus, calcium, and related renal failure function marker were elevated in the model group. Furthermore, the aortic calcium accumulation and alkaline phosphatase activity were significantly increased in the model group compared with control groups. Additionally, hematoxylin- eosin staining results demonstrated that the vascular calcification in aorta is significantly increased in the model group. Finally, the expression of VC-related proteins including bone morphogenetic protein and osteocalcin were increased in the model group, whereas alpha-smooth muscle actin was decreased in the model group compared with the control group. However, treatment with citrate caused a reversal effect of all the above events in a dose-dependent manner. In conclusion, citrate may attenuate vascular calcification in adenine-induced CRF rats.

引用

页码：452 / 458

页数：7

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