Oscillating hydrogenase reaction

The hydrogenase-catalyzed oxidation of H-2 includes an autocatalytic step in the reaction cycle. The reaction also exhibits different pH dependence in the H-2 oxidation and in the proton reduction directions. This is not only due to the pH titration of the amino acid side chains as protons are also either the substrates or the products of the reaction. Utilizing the autocatalytic nature of the hydrogenase reaction and the multiple roles of protons therein, together with appropriate limitation of the substrate (gaseous H-2) supply, oscillations can be induced in the system. The reaction oscillates both in space and in time, and can last for days with decreasing frequency until reaching chemical equilibrium. Of all biological oscillating systems described so far, this one is the simplest in that it has the fewest biological components. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.