Evaluation of three rapid assays for Mycobacterium tuberculosis complex detection in a comprehensive hospital from West China

被引：2
作者：
Hu, Xuejiao [1 ]
Shang, Mengqiao [1 ]
Chen, Xuerong [2 ]
Xie, Yi [1 ]
Ye, Yuanxin [1 ]
Zhou, Juan [1 ]
Song, Xingbo [1 ]
Lu, Xiaojun [1 ]
Ying, Binwu [1 ]
Wang, Lanlan [1 ]
机构：
[1] Sichuan Univ, West China Hosp, Dept Lab Med, Chengdu 610041, Peoples R China
[2] Sichuan Univ, West China Hosp, Dept Resp Med, Div Pulm Dis, Chengdu 610041, Peoples R China
来源：
CLINICAL BIOCHEMISTRY | 2015年 / 48卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
Tuberculosis; Diagnosis; MTD; Real-time PCR; AFB smear; REAL-TIME PCR; AMPLIFIED DIRECT TEST; SUSPECTED TUBERCULOSIS; CLINICAL-EVALUATION; SMEAR MICROSCOPY; DIAGNOSIS; PERFORMANCE; SPECIMENS; IMPACT; MTB;
D O I：
10.1016/j.clinbiochem.2014.10.003
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objectives: To assess the capacity of rapid and accurate confirmation of the Mycobacterium tuberculosis complex (MTBC) in a Chinese clinical laboratory. Design and methods: This prospective study investigated three rapid assays, the Amplified Mycobacterium Tuberculosis Direct (MTD) test, real-time PCR, and acid-fast bacilli (AFB) smear, for direct detection of MTBC in a large consecutive series of different clinical specimens. Performance parameters were estimated and compared overall and for separate specimen categories using a combined reference gold standard. Results: The overall sensitivities were similar for MTD and real-time PCR (62.26% vs. 58.49%), significantly higher than those of AFB smear (31.13%). Among three assays, MTD had a satisfactory sensitivity in respiratory specimen (73.33%) and a nearly perfect detection for smear-positive samples (96.97%). Real-time PCR showed a high positive rate (58.97%) in regard to nonrespiratory specimen. A combination of molecular assays with conventional methods reached marked additive diagnostic values (sensitivity up to 76.42%), higher than each method individually. All detection systems showed excellent specificities (>96.00%). Conclusions: The present study indicated that our lab had a moderate diagnostic performance for tuberculosis. Quality guarantee for specimen pretreatment, as well as combination analysis, will enable these assays to better incorporate into the routine laboratory workflow in China. (C) 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码：79 / 84
页数：6
相关论文
共 30 条
[1]   DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN CLINICAL SPECIMENS BY POLYMERASE CHAIN-REACTION AND GEN-PROBE AMPLIFIED MYCOBACTERIUM-TUBERCULOSIS DIRECT TEST [J].
ABE, C ;
HIRANO, K ;
WADA, M ;
KAZUMI, Y ;
TAKAHASHI, M ;
FUKASAWA, Y ;
YOSHIMURA, T ;
MIYAGI, C ;
GOTO, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (12) :3270-3274
[2]   Early detection of central nervous system tuberculosis with the gen-probe nucleic acid amplification assay: Utility in an inner city hospital [J].
Baker, CA ;
Cartwright, CP ;
Williams, DN ;
Nelson, SM ;
Peterson, PK .
CLINICAL INFECTIOUS DISEASES, 2002, 35 (03) :339-342
[3]   Clinical evaluation of the enhanced gen-probe amplified mycobacterium tuberculosis direct test for rapid diagnosis of tuberculosis in prison inmates [J].
Bergmann, JS ;
Yuoh, G ;
Fish, G ;
Woods, GL .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (05) :1419-1425
[4]   Evaluation of Cobas TaqMan MTB for Direct Detection of the Mycobacterium tuberculosis Complex in Comparison with Cobas Amplicor MTB [J].
Bloemberg, Guido V. ;
Voit, Antje ;
Ritter, Claudia ;
Deggim, Vanessa ;
Boettger, Erik C. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (07) :2112-2117
[5]   Comparison of Real Time IS6110-PCR, Microscopy, and Culture for Diagnosis of Tuberculous Meningitis in a Cohort of Adult Patients in Indonesia [J].
Chaidir, Lidya ;
Ganiem, Ahmad Rizal ;
vander Zanden, Adri ;
Muhsinin, Soni ;
Kusumaningrum, Tina ;
Kusumadewi, Inri ;
van der Ven, Andre ;
Alisjahbana, Bachti ;
Parwati, Ida ;
van Crevel, Reinout .
PLOS ONE, 2012, 7 (12)
[6]   Real-time PCR and Amplified MTD® for rapid detection of Mycobacterium tuberculosis in pulmonary specimens [J].
Chen, X. ;
Yang, Q. ;
Kong, H. ;
Chen, Y. .
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE, 2012, 16 (02) :235-239
[7]   Nucleic Acid Amplification Tests for Diagnosis of Smear-Negative TB in a High HIV-Prevalence Setting: A Prospective Cohort Study [J].
Davis, J. Lucian ;
Huang, Laurence ;
Worodria, William ;
Masur, Henry ;
Cattamanchi, Adithya ;
Huber, Charles ;
Miller, Cecily ;
Conville, Patricia S. ;
Murray, Patrick ;
Kovacs, Joseph A. .
PLOS ONE, 2011, 6 (01)
[8]   Characterization of Mycobacterium tuberculosis isolated from cancer patients with suspected tuberculosis infection in Egypt: identification, prevalence, risk factors and resistance pattern [J].
El-Sharif, A. ;
Afifi, S. ;
El-Dahshan, R. ;
Rafeh, N. ;
Eissa, S. .
CLINICAL MICROBIOLOGY AND INFECTION, 2012, 18 (11) :E438-E445
[9]   Specimen dilution improves sensitivity of the amplified Mycobacterium tuberculosis direct test for smear microscopy-Positive respiratory specimens [J].
Guerra, Renata L. ;
Baker, James F. ;
Alborz, Roya ;
Armstrong, Derek T. ;
Kiehlbauch, Julia A. ;
Conde, Marcus B. ;
Dorman, Susan E. ;
Hooper, Nancy M. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (01) :314-316
[10]   Use of the amplified Mycobacterium tuberculosis direct test in a public health laboratory -: Test performance and impact on clinical care [J].
Guerra, Renata L. ;
Hooper, Nancy M. ;
Baker, James F. ;
Alborz, Roya ;
Armstrong, Derek T. ;
Maltas, Gina ;
Kiehlbauch, Julia A. ;
Dorman, Susan E. .
CHEST, 2007, 132 (03) :946-951
123