The incorporation of strontium and zinc into a calcium-silicon ceramic for bone tissue engineering

被引：258

作者：

Zreiqat, Hala ^{[1
]}

Ramaswamy, Yogambha ^{[1
]}

Wu, Chengtie ^{[1
]}

Paschalidis, Angelo ^{[1
]}

Lu, ZuFu ^{[1
]}

James, Barbara ^{[1
]}

Birke, Oliver ^{[2
]}

McDonald, Michelle ^{[2
]}

Little, David ^{[2
]}

Dunstan, Colin R. ^{[1
]}

机构：

[1] Univ Sydney, Sch AMME, Biomat & Tissue Engn Res Unit, Sydney, NSW 2006, Australia

[2] Univ Sydney, Childrens Hosp Westmead, Sydney, NSW 2006, Australia

来源：

BIOMATERIALS | 2010年 / 31卷 / 12期

基金：

英国医学研究理事会; 澳大利亚研究理事会;

关键词：

Strontium; Zinc; CaSiO(3); Scaffolds; Bone regeneration; Orthopaedic; CELLS IN-VITRO; SIMULATED BODY-FLUIDS; BIOLOGICAL-PROPERTIES; OSTEOBLASTIC CELLS; APATITE FORMATION; CASIO3; CERAMICS; SCAFFOLDS; GLASS; VIVO; OSTEOPOROSIS;

D O I：

10.1016/j.biomaterials.2010.01.024

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this study we developed novel scaffolds through the controlled substitution and incorporation of strontium and zinc into a calcium-silicon system to form Sr-Hardystonite (Sr-Ca(2)ZnSi(2)O(7), Sr-HT). The physical and biological properties of Sr-HT were compared to Hardystonite (Ca(2)ZnSi(2)O(7)) [HT]. We showed that Sr-HT scaffolds are porous with interconnected porous network (interconnectivity: 99%) and large pore size (300-500 mu m) and an overall porosity of 78%, combined with a relatively high compressive strength (2.16 +/- 0.52 MPa). These properties are essential for enhancing bone ingrowth in load-bearing applications. Sr-HT ceramic scaffolds induced the attachment and differentiation of human bone derived cells (HOB), compared to that for the HT scaffolds. Sr-HT scaffolds enhanced expression of alkaline phosphatase, Runx-2, osteopontin, osteocalcin and bone sialoprotein. The in vivo osteoconductivity of the scaffolds was assessed at 3 and 6 weeks following implantation in tibial bone defects in rats. Histological staining revealed rapid new growth of bone into the pores of the 3D scaffolds with the Sr-HT and HT, relative to the beta-tricalcium phosphate (beta-TCP). In vivo, HT and Sr-HT produced distinct differences in the patterns of degradation of the materials, and their association with TRAP positive osteoclast-like cells with HT appearing more resistant compared to both Sr-HT and beta-TCP. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：3175 / 3184

页数：10

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