RETRACTED: EpCAM: A Potential Antimetastatic Target for Gastric Cancer (Retracted article. See vol. 58, pg. 1811, 2013)

被引：18
作者：
Du, Wenqi [1 ]
Ji, Hongzan [2 ]
Cao, Shanshan [1 ]
Wang, Li [1 ]
Bai, Feihu [1 ]
Liu, Jie [1 ,3 ]
Fan, Daiming [1 ]
机构：
[1] Fourth Mil Med Univ, Xijing Hosp, State Key Lab Canc Biol, Xian 710032, Shaanxi Prov, Peoples R China
[2] Nanjing Mil Command, PLA, Nanjing Gen Hosp, Dept Digest Dis, Nanjing 210002, Jiangsu Prov, Peoples R China
[3] Fudan Univ, Huashan Hosp, Dept Digest Dis, Shanghai 200040, Peoples R China
来源：
DIGESTIVE DISEASES AND SCIENCES | 2010年 / 55卷 / 08期
基金：
中国国家自然科学基金;
关键词：
EpCAM (epithelial cellular adhesion molecule); Gastric cancer; Metastasis; Small interfering RNA (siRNA); JUNCTION PROTEIN CLAUDIN-7; CELL ADHESION MOLECULE; MONOCLONAL-ANTIBODY; COLON-CANCER; IN-SITU; EP-CAM; EXPRESSION; METASTASIS; INVASION; CARCINOMA;
D O I：
10.1007/s10620-009-1033-8
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
Epithelial cellular adhesion molecule (EpCAM) is an attractive immunotherapeutic target to overcome metastasis of a variety of epithelium-oriented cancers. Edrecolomab, one kind of EpCAM monoclonal antibody (Panorex(A (R))), has been approved for clinical application as postoperative adjuvant therapy in breast and colorectal cancer. However, the role of EpCAM in gastric cancer metastasis remains unclear. EpCAM was found to be more highly overexpressed in metastatic gastric cancer than in nonmetastatic samples by immunohistochemistry staining. The expression level of EpCAM in gastric cancer cell lines was determined by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting, respectively. Downregulation of EpCAM by small interfering RNA (siRNA) significantly suppressed in vitro adhesive, invasive, and migratory and in vivo metastatic abilities of gastric cancer cells. We provide first evidence that EpCAM contributes to the migration of gastric cancer, suggesting that EpCAM-targeted therapy might be a promising strategy in metastatic gastric cancer.
引用
收藏
页码：2165 / 2171
页数：7
相关论文
共 23 条
[1]   EpCAM (CD326) finding its role in cancer [J].
Baeuerle, P. A. ;
Gires, O. .
BRITISH JOURNAL OF CANCER, 2007, 96 (03) :417-423
[2]   The biology of the 17-1A antigen (Ep-CAM) [J].
Balzar, M ;
Winter, MJ ;
de Boer, CJ ;
Litvinov, SV .
JOURNAL OF MOLECULAR MEDICINE-JMM, 1999, 77 (10) :699-712
[3]   Assignment of TACSTD1 (alias TROP 1, M4S1) to human chromosome 2p21 and refinement of mapping of TACSTD2 (alias TROP2, M1S1) to human chromosome 1p32 by in situ hybridization [J].
Calabrese, G ;
Crescenzi, C ;
Morizio, E ;
Palka, G ;
Guerra, E ;
Alberti, S .
CYTOGENETICS AND CELL GENETICS, 2001, 92 (1-2) :164-165
[4]   EpCAM an immunotherapeutic target for gastrointestinal malignancy: current experience and future challenges [J].
Chaudry, M. A. ;
Sales, K. ;
Ruf, P. ;
Lindhofer, H. ;
Winslet, M. C. .
BRITISH JOURNAL OF CANCER, 2007, 96 (07) :1013-1019
[5]   Effect of P-selectin monoclonal antibody on metastasis of gastric cancer and immune function [J].
Chen, JL ;
Chen, WX ;
Zhu, JS ;
Chen, NW ;
Zhou, T ;
Yao, M ;
Zhang, DQ ;
Wu, YL .
WORLD JOURNAL OF GASTROENTEROLOGY, 2003, 9 (07) :1607-1610
[6]  
Gulubova MV, 2002, HISTOCHEM J, V34, P67
[7]  
Hippo Y, 2001, CANCER RES, V61, P889
[8]   Molecular studies into the role of CD44 variants in metastasis in gastric cancer [J].
Hsieh, HF ;
Yu, JC ;
Lo, LI ;
Chiu, SC ;
Harn, HJ .
JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY, 1999, 52 (01) :25-28
[9]   Early gastric cancer in young, asymptomatic carriers of germ-line E-cadherin mutations. [J].
Huntsman, DG ;
Carneiro, F ;
Lewis, FR ;
MacLeod, PM ;
Hayashi, A ;
Monaghan, KG ;
Maung, R ;
Seruca, R ;
Jackson, CE ;
Caldas, C .
NEW ENGLAND JOURNAL OF MEDICINE, 2001, 344 (25) :1904-1909
[10]   Expression of integrin-linked kinase is closely correlated with invasion and metastasis of gastric carcinoma [J].
Ito, R ;
Oue, N ;
Zhu, XD ;
Yoshida, K ;
Nakayama, H ;
Yokozaki, H ;
Yasui, W .
VIRCHOWS ARCHIV, 2003, 442 (02) :118-123
123