Optimization of the separation of malachite green in water by capillary electrophoresis Raman spectroscopy (CE-RS) based on the stacking and sweeping modes

被引：54

作者：

Tsai, Chih-Hsin ^{[1
]}

Lin, Jia-Der ^{[1
]}

Lin, Cheng-Huang ^{[1
]}

机构：

[1] Natl Taiwan Normal Univ, Dept Chem, Taipei, Taiwan

来源：

TALANTA | 2007年 / 72卷 / 02期

关键词：

capillary electrophoresis; Raman spectroscopy; malachite green; crystal violet; stacking; sweeping;

D O I：

10.1016/j.talanta.2006.10.037

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A capillary electrophoresis Raman spectroscopy (CE-RS) method based on the stacking and sweeping modes are described. A non-fluorescent compound (malachite green, MG; crystal violet, CV) and a doubled Nd:YAG laser (532 nm, 300 mW) were selected as the model compound and light source, respectively. In order to carry out a quantitative and analysis of MG, a monochromator was used to collect the specific Raman line at 1616 cm(-1) (the N-phi and C-C stretching, corresponding to 582 nm when the wavelength of the exciting source is 532 nm). The limit of detection (LOD) for MG was 1.6 x 10(-5) and 1.1 X 10(-5) M, respectively, based on the CZE and MEKC modes. This could be improved to 3.4 x 10(-7) and 5.3 x 10(-9) M, respectively, when the stacking and sweeping modes were applied. The method was also extended to the determination of MG in an actual sample. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：368 / 372

页数：5

共 27 条

[1] On-line focusing of flavin derivatives using dynamic pH junction-sweeping capillary electrophoresis with laser-induced fluorescence detection [J].