Improvement and Interlaboratory Validation of the Lipophilic Oxygen Radical Absorbance Capacity: Determination of Antioxidant Capacities of Lipophilic Antioxidant Solutions and Food Extracts

被引：15
|
作者：
Watanabe, Jun [1 ]
Oki, Tomoyuki [2 ]
Takebayashi, Jun [3 ]
Yada, Hiroshi [1 ]
Wagaki, Manabu [1 ]
Takano-Ishikawa, Yuko [1 ]
Yasui, Akemi [1 ]
机构：
[1] Natl Agr & Food Res Org, Natl Food Res Inst, 2-1-12 Kannondai, Tsukuba, Ibaraki 3058642, Japan
[2] Natl Agr & Food Res Org, Natl Agr Res Ctr Kyushu Okinawa Reg, 2421 Suya, Koshi, Kumamoto 861192, Japan
[3] Natl Inst Hlth & Nutr, Natl Inst Biomed Innovat Hlth & Nutr, Shinjuku Ku, 1-23-1 Toyama, Tokyo 1628636, Japan
来源：
ANALYTICAL SCIENCES | 2016年 / 32卷 / 02期
关键词：
Lipophilic oxygen radical absorbance capacity; antioxidant capacity; method validation; ASSAY;
D O I：
10.2116/analsci.32.171
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A lipophilic oxygen radical absorbance capacity (L-ORAC) assay is an evaluation of the antioxidant capacity of solutions of lipophilic compounds. The concentrations of fluorescein, radical generator, and Trolox standard solutions were optimized to improve the precision of the assay. An interlaboratory study using two antioxidant solutions and three food extracts as test samples conducted in accordance with harmonized protocol demonstrated satisfactory L-ORAC measurements; the intermediate precision relative standard deviations (RSDmt) ranged from 7.0 to 16.7%, the reproducibility relative standard deviations (RSDR) ranged from 14.8 to 19.4%, and the HorRat values ranged from 1.35 to 1.78.
引用
收藏
页码：171 / 175
页数：5
相关论文
共 50 条
  • [41] A Kinetic Approach to Oxygen Radical Absorbance Capacity (ORAC): Restoring Order to the Antioxidant Activity of Hydroxycinnamic Acids and Fruit Juices
    Asma, Umme
    Bertotti, Maria Letizia
    Zamai, Simone
    Arnold, Marcellus
    Amorati, Riccardo
    Scampicchio, Matteo
    ANTIOXIDANTS, 2024, 13 (02)
  • [42] Programmable flow system for automation of oxygen radical absorbance capacity assay using pyrogallol red for estimation of antioxidant reactivity
    Ramos, Ines I.
    Gregorio, Bruno J. R.
    Barreiros, Luisa
    Magalhaes, Luis M.
    Toth, Ildiko V.
    Reis, Salette
    Lima, Jose L. F. C.
    Segundo, Marcela A.
    TALANTA, 2016, 150 : 599 - 606
  • [43] Oxygen radical antioxidant capacity (ORAC) and antibacterial properties of Melicope glabra bark extracts and isolated compounds
    Quek, Alexandra
    Zaini, Hafizah Mohd
    Kassim, Nur Kartinee
    Sulaiman, Fadzil
    Rukayadi, Yaya
    Ismail, Amin
    Abidin, Zamirah Zainal
    Awang, Khalijah
    PLOS ONE, 2021, 16 (05):
  • [44] Validation of a new method using the reactivity of electrogenerated superoxide radical in the antioxidant capacity determination of flavonoids
    Le Bourvellec, Carine
    Hauchard, Didier
    Darchen, Andre
    Burgot, Jean-Louis
    Abasq, Marie-Laurence
    TALANTA, 2008, 75 (04) : 1098 - 1103
  • [45] Optimization of ABTS radical cation assay specifically for determination of antioxidant capacity of intracellular extracts of microalgae and cyanobacteria
    Catarina Guedes, A.
    Amaro, Helena M.
    Giao, Maria S.
    Xavier Malcata, F.
    FOOD CHEMISTRY, 2013, 138 (01) : 638 - 643
  • [46] DEVELOPMENT OF AN AUTOMATED OXYGEN RADICAL ABSORBENCY CAPACITY ASSAY FOR THE DETERMINATION OF TOTAL ANTIOXIDANT ACTIVITY IN FRUITS
    WANG, H
    CAO, G
    PRIOR, RL
    FASEB JOURNAL, 1995, 9 (03): : A453 - A453
  • [47] Evaluation of Synergistic Antioxidant Potential of Complex Mixtures Using Oxygen Radical Absorbance Capacity (ORAC) and Electron Paramagnetic Resonance (EPR)
    Parker, Tory L.
    Miller, Samantha A.
    Myers, Lauren E.
    Miguez, Fernando E.
    Engeseth, Nicki J.
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (01) : 209 - 217
  • [48] Antioxidant activity of fish sauces including puffer (Lagocephalus wheeleri) fish sauce measured by the oxygen radical absorbance capacity method
    Harada, Kazuki
    Maeda, Toshimichi
    Hasegawa, Yoshiro
    Tokunaga, Takushi
    Tamura, Yoshiyuki
    Koizumi, Takeo
    MOLECULAR MEDICINE REPORTS, 2010, 3 (04) : 663 - 668
  • [49] Dietary intake, neutrophil fatty acid profile, serum antioxidant vitamins and oxygen radical absorbance capacity in patients with ulcerative colitis
    Kawakami, Yuko
    Okada, Hiroyuki
    Murakami, Yasuko
    Kawakami, Takayo
    Ueda, Yukiko
    Kunii, Daisuke
    Sakamoto, Yachiyo
    Shiratori, Yasushi
    Okita, Misako
    JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 2007, 53 (02) : 153 - 159
  • [50] Comparative Evaluation of 2,2-Dipheny1-1-Picryl-Hydrazylhydrate (DPPH) Free Radical- and Oxygen Radical Absorbance Capacity (ORAC) Assays in Measuring the Antioxidant Capacities of Pigmented Rice Varieties
    Chen, Xiaoqiong
    Irifune, Kohei
    Yang, Dingqian
    Nagao, Norio
    Chikawa, Yuuki
    Wu, Xianjun
    Itani, Tomio
    PHILIPPINE AGRICULTURAL SCIENTIST, 2017, 100 (02): : 190 - 199
12345