Pathways to defense metabolites and evading fruit bitterness in genus Solanum evolved through 2-oxoglutarate-dependent dioxygenases

被引：82

作者：

Cardenas, Pablo D. ^{[1
,2
,3
,12
]}

Sonawane, Prashant D. ^{[1
]}

Heinig, Uwe ^{[1
]}

Jozwiak, Adam ^{[1
]}

Panda, Sayantan ^{[1
]}

Abebie, Bekele ^{[4
]}

Kazachkova, Yana ^{[1
]}

Pliner, Margarita ^{[1
]}

Unger, Tamar ^{[5
]}

Wolf, Dalia ^{[6
]}

Ofner, Itai ^{[7
]}

Vilaprinyo, Ester ^{[8
]}

Meir, Sagit ^{[1
]}

Davydov, Olga ^{[1
]}

Gal-On, Amit ^{[4
]}

Burdman, Saul ^{[2
,3
]}

Giri, Ashok ^{[9
]}

Zamir, Dani ^{[7
]}

Scherf, Tali ^{[10
]}

Szymanski, Jedrzej ^{[11
]}

Rogachev, Ilana ^{[1
]}

Aharoni, Asaph ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Plant & Environm Sci, IL-7610001 Rehovot, Israel

[2] Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, Dept Plant Pathol & Microbiol, IL-7610001 Rehovot, Israel

[3] Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, Otto Warburg Minerva Ctr Agr Biotechnol, IL-7610001 Rehovot, Israel

[4] Agr Res Org, Volcani Ctr, Dept Plant Pathol & Weed Res, IL-7505101 Rishon Leziyyon, Israel

[5] Weizmann Inst Sci, Israel Struct Prote Ctr, IL-7610001 Rehovot, Israel

[6] Agr Res Org, Volcani Ctr, Dept Vegetable Res, IL-7505101 Rishon Leziyyon, Israel

[7] Hebrew Univ Jerusalem, Inst Plant Sci & Genet Agr, Robert H Smith Fac Agr Food & Environm, IL-7610001 Rehovot, Israel

[8] Univ Lleida, Dept Basic Med Sci, IRBLleida, Lleida, Spain

[9] CSIR, Natl Chem Lab, Plant Mol Biol Unit, Div Biochem Sci, Pune 411008, Maharashtra, India

[10] Weizmann Inst Sci, Dept Chem Res Support, NMR Unit, IL-7610001 Rehovot, Israel

[11] Leibniz Inst Plant Genet & Crop Plant Res, OT Gatersleben, Dept Mol Genet, Corrensstr 3, D-06466 Seeland, Germany

[12] Univ Copenhagen, Dept Plant & Environm Sci, Fac Sci, Frederiksberg C, Denmark

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

欧盟地平线“2020”; 以色列科学基金会; 欧洲研究理事会;

关键词：

COLORADO POTATO BEETLE; LEPTINOTARSA-DECEMLINEATA SAY; LEPTINE GLYCOALKALOIDS; PLANT; IDENTIFICATION; SOLASODINE; RESISTANCE; BIOSYNTHESIS; SEGREGATION; POPULATION;

D O I：

10.1038/s41467-019-13211-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The genus Solanum comprises three food crops (potato, tomato, and eggplant), which are consumed on daily basis worldwide and also producers of notorious anti-nutritional steroidal glycoalkaloids (SGAs). Hydroxylated SGAs (i.e. leptinines) serve as precursors for leptines that act as defenses against Colorado Potato Beetle (Leptinotarsa decemlineata Say), an important pest of potato worldwide. However, SGA hydroxylating enzymes remain unknown. Here, we discover that 2-OXOGLUTARATE-DEPENDENT-DIOXYGENASE (2-ODD) enzymes catalyze SGA-hydroxylation across various Solanum species. In contrast to cultivated potato, Solanum chacoense, a widespread wild potato species, has evolved a 2-ODD enzyme leading to the formation of leptinines. Furthermore, we find a related 2-ODD in tomato that catalyzes the hydroxylation of the bitter a-tomatine to hydroxytomatine, the first committed step in the chemical shift towards downstream ripening-associated non-bitter SGAs (e.g. esculeoside A). This 2-ODD enzyme prevents bitterness in ripe tomato fruit consumed today which otherwise would remain unpleasant in taste and more toxic.

引用

页数：13

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