Label-Free Fluorescence Spectroscopy for Detecting Key Biomolecules in Brain Tissue from a Mouse Model of Alzheimer's Disease

被引：39
作者：
Shi, Lingyan [1 ,3 ,6 ]
Lu, Luyao [2 ]
Harvey, George [5 ]
Harvey, Thomas [5 ]
Rodriuez-Contreras, Adrian [1 ,3 ]
Alfano, Robert R. [1 ,4 ]
机构：
[1] CUNY City Coll, Inst Ultrafast Spect & Lasers, New York, NY 10031 USA
[2] Wenzhou Med Univ, Dept Biomed Engn, Wenzhou, Zhejiang, Peoples R China
[3] CUNY City Coll, Dept Biol, 138th St & Convent Ave, New York, NY 10031 USA
[4] CUNY City Coll, Dept Phys, New York, NY 10031 USA
[5] Riverdale Country Sch, Bronx, NY USA
[6] Columbia Univ, Dept Chem, New York, NY 10027 USA
来源：
SCIENTIFIC REPORTS | 2017年 / 7卷
基金：
中国国家自然科学基金;
关键词：
DYSFUNCTION; BETA; TAU;
D O I：
10.1038/s41598-017-02673-5
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In this study, label-free fluorescence spectroscopy was used for the first time to determine spectral profiles of tryptophan, reduced nicotinamide adenine dinucleotide (NADH), and flavin denine dinucleotide (FAD) in fresh brain samples of a mouse model of Alzheimer's disease (AD). Our results showed that the emission spectral profile levels of tryptophan and NADH were higher in AD samples than normal samples. The intensity ratio of tryptophan to NADH and the change rate of fluorescence intensity with respect to wavelength also increased in AD brain. These results yield an optical method for detecting early stage of AD by comparing spectral profiles of biomolecules.
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页数：7
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