Isolation of pisumin, a novel antifungal protein from legumes of the sugar snap pea Pisum sativum var. macrocarpon

被引：8
|
作者：
Ye, XY [1 ]
Ng, TB [1 ]
机构：
[1] Chinese Univ Hong Kong, Fac Med, Dept Biochem, Shaun, Hong Kong, Peoples R China
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2003年 / 134卷 / 02期
关键词：
antifungal protein; sugar snap pea; legume; Pisum sativum; isolation; translation-inhibitory; characterization;
D O I：
10.1016/S1532-0456(02)00251-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An antifungal protein with a novel N-terminal sequence GVGAAYGCFG and a molecular mass of 31 kDa was isolated from the legumes of the sugar snap pea Pisum sativum var. macrocarpon. The protein, designated pisumin, exhibited antifungal activity against Coprinus comatus and Pleurotus ostreatus and much weaker activity against Fusarium oxysporum and Rhizoctonia solani. Pisumin inhibited cell-free translation in a rabbit reticulocyte lysate system with an IC50 of 6 muM. Pisumin was similar to other leguminous antifungal proteins in that it was adsorbed on Affi-gel blue gel and CM-Sepharose. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：235 / 240
页数：6
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