No DNA damage response and negligible genome-wide transcriptional changes in human embryonic stem cells exposed to terahertz radiation

被引：71

作者：

Bogomazova, A. N. ^{[1
,6
]}

Vassina, E. M. ^{[1
,6
]}

Goryachkovskaya, T. N. ^{[2
]}

Popik, V. M. ^{[3
]}

Sokolov, A. S. ^{[4
]}

Kolchanov, N. A. ^{[2
]}

Lagarkova, M. A. ^{[1
,5
,6
]}

Kiselev, S. L. ^{[1
,6
]}

Peltek, S. E. ^{[2
]}

机构：

[1] RAS, Vavilov Inst Gen Genet, Moscow 117901, Russia

[2] RAS, Inst Cytol & Genet, Novosibirsk, Russia

[3] RAS, Budker Inst Nucle Phys, SB, Novosibirsk, Russia

[4] RAS, Ctr Bioengn, Moscow 117901, Russia

[5] Sci Res Inst Phys Chem Med, Moscow, Russia

[6] Skolkovo Inst Sci & Technol, Skoltech Ctr Stem Cell Res, Skolkovo, Moscow Region, Russia

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

俄罗斯基础研究基金会;

关键词：

DOUBLE-STRAND BREAKS; THZ; EXPRESSION; GENES;

D O I：

10.1038/srep07749

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Terahertz (THz) radiation was proposed recently for use in various applications, including medical imaging and security scanners. However, there are concerns regarding the possible biological effects of non-ionising electromagnetic radiation in the THz range on cells. Human embryonic stem cells (hESCs) are extremely sensitive to environmental stimuli, and we therefore utilised this cell model to investigate the non-thermal effects of THz irradiation. We studied DNA damage and transcriptome responses in hESCs exposed to narrow-band THz radiation (2.3 THz) under strict temperature control. The transcription of approximately 1% of genes was subtly increased following THz irradiation. Functional annotation enrichment analysis of differentially expressed genes revealed 15 functional classes, which were mostly related to mitochondria. Terahertz irradiation did not induce the formation of gamma H2AX foci or structural chromosomal aberrations in hESCs. We did not observe any effect on the mitotic index or morphology of the hESCs following THz exposure.

引用

页数：6

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