Simple diagnosis of HbAlc using the dual-plasmonic platform integrated with LSPR and SERS

被引：9

作者：

Heo, Nam Su ^{[1
]}

Kwak, Cheol Hwan ^{[1
]}

Lee, Hoomin ^{[1
]}

Kim, Dongjoo ^{[1
]}

Lee, Sunmook ^{[2
]}

Kim, Gi-bum ^{[3
]}

Kwon, Soonjo ^{[1
]}

Kim, Woo Sik ^{[4
]}

Huh, Yun Suk ^{[1
]}

机构：

[1] Inha Univ, Dept Biol Engn, Incheon 22212, South Korea

[2] Korea Conform Labs, Adv Mat Reliabil Assessment Ctr, Seoul 08503, South Korea

[3] Room 1-1302,16-4,Dongbaekjungang Ro 16Beon Gil, Yongin 17015, Gyeonggi Do, South Korea

[4] Kyung Hee Univ, Dept Chem Engn, Coll Engn, Yongin 17104, South Korea

来源：

JOURNAL OF CRYSTAL GROWTH | 2017年 / 469卷

基金：

新加坡国家研究基金会;

关键词：

Glycated hemoglobin; Localized surface plasmon resonance; Surface-enhanced Raman spectroscopy; Gold nanoparticles; Self-assembled method; Biosensor; GLYCATED HEMOGLOBIN; RAMAN-SPECTROSCOPY; SURFACE; RESONANCE; HBA(1C); DISEASE; NANOPARTICLES; RISK; ARTERIAL; COMPLEX;

D O I：

10.1016/j.jcrysgro.2016.09.039

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

A plasmonic active chip was designed with a transparent polymer film self-assembled with gold nanoparticles (AuNPs). In this study, we demonstrated the feasibility and sensitivity of biosensors by employing a plasmonic resonance technique. AuNPs are widely used as biosensing probes because they facilitate stable immobilization of biomolecules. Transparent polymer film facilitated measurement of changes in absorbance via transmitted light and analysis of Raman scattering via scattered light. The cysteine rich protein G and anti-HbAlc were sequentially conjugated to self-assembled AuNPs on the transparent polymer film to detect a target protein. HbAlc, which is used as an indicator for diabetes diagnosis, was selected for target protein detection. We confirmed the linearly increased absorbance values with increasing HbAlc level (3.19-14.0%) by LSPR detection. We also verified the linear increase in SERS intensity as the concentration of anti-Hb increased from 10 ng mL(-1) to 1 mu g mL(-1) by analyzing the SERS spectra of Cy3 labeled anti-Hb added substrates.

引用

页码：154 / 159

页数：6

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