A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting

被引：8

作者：

Akuta, Teruo ^{[1
]}

Maruyama, Toshiaki ^{[2
]}

Sakuma, Chiaki ^{[1
]}

Nakagawa, Masataka ^{[1
]}

Tomioka, Yui ^{[1
]}

Entzminger, Kevin ^{[2
]}

Fleming, Jonathan K. ^{[2
]}

Sato, Ryo ^{[1
]}

Shibata, Takashi ^{[1
]}

Kurosawa, Yasunori ^{[1
,2
]}

Okumura, C. J. ^{[2
]}

Arakawa, Tsutomu ^{[3
]}

机构：

[1] Kyokuto Pharmaceut Ind Co Ltd, Res & Dev Div, 3333-26 Aza Asayama, Takahagi, Ibaraki 3180004, Japan

[2] Abwiz Bio Inc, 9823 Pacific Hts Blvd,Suite J, San Diego, CA 92121 USA

[3] Alliance Prot Labs, 13380 Pantera Rd, San Diego, CA 92130 USA

来源：

ANTIBODIES | 2022年 / 11卷 / 02期

关键词：

native gel; blotting; conformation-specific; antibody; SARS-CoV-2; conformation-indifferential; HUMAN CYSTATIN-C; POLYACRYLAMIDE GELS; PROTEINS; DIFFUSION; OLIGOMERS;

D O I：

10.3390/antib11020036

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the native state of these structures. Green fluorescent protein showed functional state both on agarose gel and blotted membrane. Based on the combined procedures, we discovered conformation-specific monoclonal antibodies against PLXDC2 and SARS-CoV-2 spike protein.

引用

页数：17

共 23 条

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Sakuma, Chiaki
Sato, Tomomi
Shibata, Takashi
Nakagawa, Masataka
Kurosawa, Yasunori
Okumura, C. J.
Maruyama, Toshiaki
Arakawa, Tsutomu
Akuta, Teruo
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