High-resolution immunofluorescence imaging of mouse cochlear hair bundles

被引：3
作者：
Miller, Katharine K. [1 ]
Wang, Pei [1 ]
Grillet, Nicolas [1 ]
机构：
[1] Stanford Univ, Dept Otolaryngol Head & Neck Surg, Stanford, CA 94305 USA
来源：
STAR PROTOCOLS | 2022年 / 3卷 / 02期
关键词：
MECHANOTRANSDUCTION MACHINERY; STEREOCILIA; CHANNEL; CELLS; TRANSDUCTION; COMPONENT; LINKS; TMIE;
D O I：
10.1016/j.xpro.2022.101431
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-resolution immunofluorescence imaging of cochlear hair bundles faces many challenges due to the hair bundle's small dimensions, fragile nature, and complex organization. Here, we describe an optimized protocol for hair-bundle protein immunostaining and localization. We detail the steps and solutions for extracting and fixing the mouse inner ear and for dissecting the organ of Corti. We further emphasize the optimal permeabilization, blocking, staining, and mounting conditions as well as the parameters for high-resolution microscopy imaging.For complete details on the use and execution of this protocol, please refer to Trouillet et al. (2021).
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页数：22
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