Molecular characterization and gene expression of the pituitary adenylate cyclase-activating polypeptide (PACAP) in the lizard brain

被引：15
|
作者：
Valiante, Salvatore [1 ]
Prisco, Marina [1 ]
Capaldo, Anna [1 ]
Zambrano, Immacolata [1 ]
De Falco, Maria [1 ]
Andreuccetti, Piero [1 ]
Laforgia, Vincenza [1 ]
Varano, Lorenzo [1 ]
机构：
[1] Univ Naples Federico II, Dipartimento Biol Evolut & Comparata, I-80134 Naples, Italy
来源：
BRAIN RESEARCH | 2007年 / 1127卷 / 01期
关键词：
regulatory peptide; in situ hybridization; reptile; T-PCR; sequence analysis; evolution;
D O I：
10.1016/j.brainres.2006.09.103
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The pituitary adenylate cyclase-activating polypeptide (PACAP) is considered a pleiotropic neuropeptide in vertebrate physiology. The nucleotide sequence, the expression and the distribution of PACAP were determined in the brain of the lizard Podarcis sicula. RT-PCR showed that the brain of this reptile synthesizes an mRNA coding for PACAP. By performing in situ hybridization and immunohistochemistry techniques, a wide distribution of PACAP and its mRNA in neurons, nervous fibers and other cells was found. Phylogenetic sequence analysis indicates that lizard PACAP is highly conserved, resembling the vertebrate PACAP. Our data demonstrate that PACAP is not only highly preserved during vertebrate evolution but also suggest that PACAP could be implicated in a wide number of functions in the physiology of the reptile brain. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：66 / 75
页数：10
相关论文
共 50 条
  • [41] SEQUENCE AND EXPRESSION OF CDNA FOR PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) AND GROWTH HORMONE-RELEASING HORMONE (GHRH)-LIKE PEPTIDE IN CATFISH
    MCRORY, JE
    PARKER, DB
    NGAMVONGCHON, S
    SHERWOOD, NM
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1995, 108 (1-2) : 169 - 177
  • [42] Chronic stress increases pituitary adenylate cyclase-activating peptide (PACAP) and brain-derived neurotrophic factor (BDNF) mRNA expression in the bed nucleus of the stria terminalis (BNST): Roles for PACAP in anxiety-like behavior
    Hammack, Sayamwong E.
    Cheung, Joseph
    Rhodes, Kimberly M.
    Schutz, Kristin C.
    Falls, William A.
    Braas, Karen M.
    May, Victor
    PSYCHONEUROENDOCRINOLOGY, 2009, 34 (06) : 833 - 843
  • [43] PITUITARY ADENYLATE-CYCLASE ACTIVATING PEPTIDE (PACAP) MESSENGER-RNA IN THE RAT NEOCORTEX
    MIKKELSEN, JD
    HANNIBAL, J
    LARSEN, PJ
    FAHRENKRUG, J
    NEUROSCIENCE LETTERS, 1994, 171 (1-2) : 121 - 124
  • [44] Pituitary adenylate cyclase activating polypeptide (PACAP) and its receptor 1 (PAC1) in the human infant brain and changes in the Sudden Infant Death Syndrome (SIDS)
    Huang, J.
    Waters, K. A.
    Machaalani, R.
    NEUROBIOLOGY OF DISEASE, 2017, 103 : 70 - 77
  • [45] Differential regulation of pituitary adenylate cyclase-activating peptide receptor variants in the rat suprachiasmatic nucleus
    Shinohara, K
    Funabashi, T
    Nakamura, TJ
    Mitsushima, D
    Kimura, F
    NEUROSCIENCE, 2002, 110 (02) : 301 - 308
  • [46] Embryonic expression of pituitary adenylyl cyclase-activating polypeptide and its selective type I receptor gene in the frog Xenopus laevis neural tube
    Hu, ZT
    Lelievre, V
    Rodriguez, WI
    Tam, J
    Cheng, JW
    Cohen-Cory, S
    Waschek, JA
    JOURNAL OF COMPARATIVE NEUROLOGY, 2001, 441 (03) : 266 - 275
  • [47] Reduced expression of brain-derived neurotrophic factor in mice deficient for pituitary adenylate cyclase activating polypeptide type-I-receptor
    Zink, M
    Otto, C
    Zörner, B
    Zacher, C
    Schütz, G
    Henn, FA
    Gass, P
    NEUROSCIENCE LETTERS, 2004, 360 (1-2) : 106 - 108
  • [48] Hypoxia and nicotine effects on Pituitary adenylate cyclase activating polypeptide (PACAP) and its receptor 1 (PAC1) in the developing piglet brainstem
    Huang, J.
    Waters, K. A.
    Machaalani, R.
    NEUROTOXICOLOGY, 2017, 62 : 30 - 38
  • [49] Induction of pituitary adenylate cyclase-activating polypeptide mRNA in the medial parvocellular part of the paraventricular nucleus of rats following kainic-acid-induced seizure
    Nomura, M
    Ueta, Y
    Hannibal, J
    Serino, R
    Yamamoto, Y
    Shibuya, I
    Matsumoto, T
    Yamashita, H
    NEUROENDOCRINOLOGY, 2000, 71 (05) : 318 - 326
  • [50] Developmental Changes in Pituitary Adenylate Cyclase Activating Polypeptide Expression during the Perinatal Period: Possible Role in Fetal Gonadotroph Regulation
    Moore, Joseph P., Jr.
    Villafuerte, Betty C.
    Unick, Christian A.
    Winters, Stephen J.
    ENDOCRINOLOGY, 2009, 150 (10) : 4802 - 4809
12345