Molecular characterization and gene expression of the pituitary adenylate cyclase-activating polypeptide (PACAP) in the lizard brain

被引：15
|
作者：
Valiante, Salvatore [1 ]
Prisco, Marina [1 ]
Capaldo, Anna [1 ]
Zambrano, Immacolata [1 ]
De Falco, Maria [1 ]
Andreuccetti, Piero [1 ]
Laforgia, Vincenza [1 ]
Varano, Lorenzo [1 ]
机构：
[1] Univ Naples Federico II, Dipartimento Biol Evolut & Comparata, I-80134 Naples, Italy
来源：
BRAIN RESEARCH | 2007年 / 1127卷 / 01期
关键词：
regulatory peptide; in situ hybridization; reptile; T-PCR; sequence analysis; evolution;
D O I：
10.1016/j.brainres.2006.09.103
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The pituitary adenylate cyclase-activating polypeptide (PACAP) is considered a pleiotropic neuropeptide in vertebrate physiology. The nucleotide sequence, the expression and the distribution of PACAP were determined in the brain of the lizard Podarcis sicula. RT-PCR showed that the brain of this reptile synthesizes an mRNA coding for PACAP. By performing in situ hybridization and immunohistochemistry techniques, a wide distribution of PACAP and its mRNA in neurons, nervous fibers and other cells was found. Phylogenetic sequence analysis indicates that lizard PACAP is highly conserved, resembling the vertebrate PACAP. Our data demonstrate that PACAP is not only highly preserved during vertebrate evolution but also suggest that PACAP could be implicated in a wide number of functions in the physiology of the reptile brain. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：66 / 75
页数：10
相关论文
共 50 条
  • [31] Tracing the Origins of the Pituitary Adenylate-Cyclase Activating Polypeptide (PACAP) (vol 14, 366, 2020)
    Cardoso, Joao C. R.
    Garcia, Manuel G.
    Power, Deborah M.
    FRONTIERS IN NEUROSCIENCE, 2020, 14
  • [32] Pituitary adenylate cyclase-activating polypeptide does not colocalize with vasoactive intestinal polypeptide in the hypothalamic magnocellular nuclei and posterior pituitary of cats and rats
    Vereczki, V
    Köves, K
    Tóth, ZE
    Baba, A
    Hashimoto, H
    Fógel, K
    Arimura, A
    Kausz, M
    ENDOCRINE, 2003, 22 (03) : 225 - 237
  • [33] Targeted Pituitary Adenylate Cyclase-Activating Peptide Therapies for Migraine
    Vollesen, Anne Luise Haulund
    Amin, Faisal Mohammad
    Ashina, Messoud
    NEUROTHERAPEUTICS, 2018, 15 (02) : 371 - 376
  • [34] Pituitary adenylate cyclase activating peptide (PACAP) immunoreactivity and mRNA expression in the duck gastrointestinal tract
    Mirabella, N
    Squillacioti, C
    Colitti, M
    Germano, G
    Pelagalli, A
    Paino, G
    CELL AND TISSUE RESEARCH, 2002, 308 (03) : 347 - 359
  • [35] Pituitary adenylate cyclase-activating polypeptide and islet amyloid polypeptide in primary sensory neurons - Functional implications from plasticity in expression on nerve injury and inflammation
    Mulder, H
    Jongsma, H
    Zhang, YZ
    Gebre-Medhin, S
    Sundler, F
    Danielsen, N
    MOLECULAR NEUROBIOLOGY, 1999, 19 (03) : 229 - 253
  • [36] Increased pituitary adenylate cyclase-activating polypeptide in the central bed nucleus of the stria terminalis in mood disorders in men
    Slabe, Zala
    Pechler, Gwyneth A.
    van Heerikhuize, Joop
    Samuels, Benjamin A.
    Zivin, Marko
    Zorovic, Maja
    Swaab, Dick F.
    NEUROBIOLOGY OF DISEASE, 2023, 183
  • [37] Pharmacology and functions of receptors for vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide: IUPHAR Review 1
    Harmar, Anthony J.
    Fahrenkrug, Jan
    Gozes, Illana
    Laburthe, Marc
    May, Victor
    Pisegna, Joseph R.
    Vaudry, David
    Vaudry, Hubert
    Waschek, James A.
    Said, Sami I.
    BRITISH JOURNAL OF PHARMACOLOGY, 2012, 166 (01) : 4 - 17
  • [38] Pituitary adenylate cyclase-activating peptide is upregulated in sensory neurons by inflammation
    Zhang, YZ
    Danielsen, N
    Sundler, F
    Mulder, H
    NEUROREPORT, 1998, 9 (12) : 2833 - 2836
  • [39] PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE IS EXPRESSED IN AUTONOMIC NEURONS
    MULDER, H
    UDDMAN, R
    MOLLER, K
    ELSAS, T
    EKBLAD, E
    ALUMETS, J
    SUNDLER, F
    REGULATORY PEPTIDES, 1995, 59 (01) : 121 - 128
  • [40] Roles for Pituitary Adenylate Cyclase-Activating Peptide (PACAP) Expression and Signaling in the Bed Nucleus of the Stria Terminalis (BNST) in Mediating the Behavioral Consequences of Chronic Stress
    Hammack, Sayamwong E.
    Roman, Carolyn W.
    Lezak, Kimberly R.
    Kocho-Shellenberg, Margaret
    Grimmig, Bethany
    Falls, William A.
    Braas, Karen
    May, Victor
    JOURNAL OF MOLECULAR NEUROSCIENCE, 2010, 42 (03) : 327 - 340
12345