Molecular characterization and gene expression of the pituitary adenylate cyclase-activating polypeptide (PACAP) in the lizard brain

被引：15
|
作者：
Valiante, Salvatore [1 ]
Prisco, Marina [1 ]
Capaldo, Anna [1 ]
Zambrano, Immacolata [1 ]
De Falco, Maria [1 ]
Andreuccetti, Piero [1 ]
Laforgia, Vincenza [1 ]
Varano, Lorenzo [1 ]
机构：
[1] Univ Naples Federico II, Dipartimento Biol Evolut & Comparata, I-80134 Naples, Italy
来源：
BRAIN RESEARCH | 2007年 / 1127卷 / 01期
关键词：
regulatory peptide; in situ hybridization; reptile; T-PCR; sequence analysis; evolution;
D O I：
10.1016/j.brainres.2006.09.103
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The pituitary adenylate cyclase-activating polypeptide (PACAP) is considered a pleiotropic neuropeptide in vertebrate physiology. The nucleotide sequence, the expression and the distribution of PACAP were determined in the brain of the lizard Podarcis sicula. RT-PCR showed that the brain of this reptile synthesizes an mRNA coding for PACAP. By performing in situ hybridization and immunohistochemistry techniques, a wide distribution of PACAP and its mRNA in neurons, nervous fibers and other cells was found. Phylogenetic sequence analysis indicates that lizard PACAP is highly conserved, resembling the vertebrate PACAP. Our data demonstrate that PACAP is not only highly preserved during vertebrate evolution but also suggest that PACAP could be implicated in a wide number of functions in the physiology of the reptile brain. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：66 / 75
页数：10
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