VE-cadherin-CreERT2 transgenic mouse:: A model for inducible recombination in the endothelium

被引：197
作者：
Monvoisin, Arnaud
Alva, Jackelyn A.
Hofmann, Jennifer J.
Zovein, Ann C.
Lane, Timothy F.
Iruela-Arispe, M. Luisa
机构：
[1] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Dept Mol Cellular & Dev Biol, Los Angeles, CA USA
来源：
DEVELOPMENTAL DYNAMICS | 2006年 / 235卷 / 12期
关键词：
endothelium; VE-cadherin; Cadherin-5; Cre-recombinase; tamoxifen; transgenic mice; tumor angiogenesis;
D O I：
10.1002/dvdy.20982
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
To introduce temporal control in genetic experiments targeting the endothelium, we established a mouse line expressing tamoxifen-inducible Cre-recombinase (Cre-ERT2) under the regulation of the vascular endothelial cadherin promoter (VECad). Specificity and efficiency of Cre activity was documented by crossing VECad-Cre-ERT2 with the ROSA26R reporter mouse, in which a floxed-stop cassette has been placed upstream of the beta-galactosidase gene. We found that tamoxifen specifically induced widespread recombination in the endothelium of embryonic, neonatal, and adult tissues. Recombination was also documented in tumor-associated vascular beds and in postnatal angiogenesis assays. Furthermore, injection of tamoxifen in adult animals resulted in negligible excision (lower than 0.4%) in the hematopoietic lineage. The VECad-Cre-ERT2 Mouse is likely to be a valuable tool to study the function of genes involved in vascular development, homeostasis, and in complex processes involving neoangiogenesis, such as tumor growth.
引用
收藏
页码：3413 / 3422
页数：10
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