Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification

被引：23
作者：
Ren, Chun-Hua
Hu, Chao-Qun [1 ]
Luo, Peng
Wang, Qing-Bai
机构：
[1] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China
来源：
MICROBIOLOGICAL RESEARCH | 2009年 / 164卷 / 05期
关键词：
Identification; Vibrio vulnificus; LAMP; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; DNA; LAMP; OYSTERS; DIFFERENTIATION; QUANTIFICATION; DIAGNOSIS; PATHOGEN; WATER;
D O I：
10.1016/j.micres.2008.05.002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Vibrio vulnificus is a serious bacterial. pathogen for humans and aquatic animals. We developed a rapid, sensitive and specific identification method for V vulnificus using loop-mediated isothermal amplification (LAMP) technique. A set of primers, composed of two outer primers and two inner primers, was designed based on the cytolysin gene sequence of V. vulnificus. The LAMP reaction was processed in a heat block at 65 degrees C for 60 min. The amplification products were detected by visual inspection using SYBR Green 1, as welt as by electrophoresis on agarose gets. Our results showed that the LAMP reaction was highly specific to V. vulnificus. This method was 10-fold more sensitive than conventional PCR. In conclusion, the LAMP assay was extremely rapid, simple, cost-effective, sensitive and specific for the rapid identification of V. vulnificus. (C) 2008 Elsevier GmbH. All rights reserved.
引用
收藏
页码：514 / 521
页数：8
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