Towards improving ELISA surfaces: SPR assessment of polystyrene modification efficiency for promoting immobilization of biomolecules

被引：8

作者：

Beketov, Gennadii ^{[1
]}

Shynkarenko, Olena ^{[1
]}

Apatska, Mariia ^{[1
]}

机构：

[1] NAS Ukraine, VE Lashkaryov Inst Semicond Phys, Nauki Ave 41, UA-03028 Kiev, Ukraine

来源：

ANALYTICAL BIOCHEMISTRY | 2021年 / 618卷

关键词：

ELISA Enzyme-linked immunosorbent assay; Surface plasmon resonance; Polystyrene; Surface modification; Biomolecule immobilization;

D O I：

10.1016/j.ab.2021.114101

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzyme-linked immunosorbent assay (ELISA) is a widely used diagnostic technique. In ELISA, detection of the target biomolecules is achieved through selective capture by appropriate antibody immobilized on a solid support. Our study addresses the application of surface plasmon resonance to an assessment of the polystyrene modification efficiency for promoting adsorption of biomolecules. A method facilitating the development of advanced immobilization strategies for biofunctionalization of polystyrene surface was evolved. The proposed approach uses formation of a thin layer of polystyrene over the SPR chip surface, thus enabling a detailed characterization of biomolecular interactions at the polystyrene surface.

引用

页数：5

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