Effects of low-molecular-weight polyols on the hydration status of the light-harvesting complex 2 from Rhodobacter sphaeroides 2.4.1

被引:1
作者
Shi, Ying [1 ]
Yu, Jie [3 ]
Liu, Yu-Chen [4 ]
Wang, Peng [2 ]
Zhang, Jian-Ping [2 ]
机构
[1] Baotou Teachers Coll, Dept Chem, Baotou 014030, Peoples R China
[2] Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China
[3] Weifang Med Univ, Coll Pharm, Weifang 261042, Peoples R China
[4] Lund Univ, Div Phys Chem, Dept Chem, Box 124, S-22100 Lund, Sweden
关键词
Rhodobacter sphaeroides; Hydration; Glycerol; Sorbitol; Triplet excited state;
D O I
10.1007/s43630-021-00046-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Low-molecular-weight (MW) polyols are organic osmolytes influencing water activity. We have investigated the effects of polyol molecules (glycerol and sorbitol) on the optical and triplet excitation dynamics of light-harvesting complex 2 (LH2) from Rhodobacter (Rba.) sphaeroides in buffer-detergent solutions. The resonance Raman spectroscopy demonstrated that, on increasing glycerol and sorbitol volume fractions ranging from 0 to 80% (v/v) (accompanied by the decreasing water activities), the planar and all-trans conformation of carotenoids (Crts) remained unchanged, and the bacteriochlorophyll a (BChl) Q(y) absorption intensity decreased. The B850 fluorescence amplitude elevated in the 20-80% v/v sorbitol and 20-40% v/v glycerol solution, but decreased in 80% v/v glycerol solution. The change of (3)[Crt*-BChl] interaction bands caused by (3)Crt*-BChl interaction had no obvious correlation with water activities against polyol volume fractions, which are rationalized by the water activity sensitive of C- and N-termini of protein which binding with BChls. The results suggest that Rba. sphaeroides LH2 is more sensitive to low-molecular-weight polyols compared with that of the thermophiles purple bacterium Thermochromatium (Tch.) tepidum we had investigated before. Graphic abstract
引用
收藏
页码:627 / 637
页数:11
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