Multiplex enzyme immunoassay system for the simultaneous detection of multiple allergens in foods

被引：34

作者：

Blais, BW ^{[1
]}

Gaudreault, M ^{[1
]}

Phillippe, LM ^{[1
]}

机构：

[1] Canadian Food Inspect Agcy, Lab Serv Div, CEF, Ottawa, ON K1A 0C6, Canada

来源：

FOOD CONTROL | 2003年 / 14卷 / 01期

关键词：

multiplex; enzyme immunoassay; allergens; peanut; hazelnut; Brazil nut;

D O I：

10.1016/S0956-7135(02)00053-1

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

An enzyme immunoassay (EIA) system was developed for the simultaneous detection of multiple allergens in foods. This assay utilized a reverse dot blot EIA format in which egg yolk antibodies (IgY) specific for different allergens were immobilized in discrete spots on a strip of polyester cloth, which was then contacted with the test sample. The binding of antigens to a specific spot on the strip was revealed by sequential reactions with a pool of biotinylated allergen-specific antibodies, streptavidin-peroxidase conjugate and a chromogenic substrate. As an example of the applicability of this multiplex EIA system, we demonstrate the detection of three important food allergens: peanut, hazelnut and Brazil nut. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：43 / 47

页数：5

共 13 条

[1] Determination of peanut traces in food by a commercially-available ELISA test [J].

Keck-Gassenmeier, B ;

Bénet, S ;

Rosa, C ;

Hischenhuber, C .

FOOD AND AGRICULTURAL IMMUNOLOGY, 1999, 11 (03) :243-250

[2] Polymacron enzyme immunoassay system for detection of naturally contaminating Salmonella in foods, feeds, and environmental samples [J].

Blais, BW ;

Pietrzak, E ;

Oudit, D ;

Wilson, C ;

Phillippe, LM ;

Howlett, J .

JOURNAL OF FOOD PROTECTION, 1998, 61 (09) :1187-1190

[3] Detection of hazelnut proteins in foods by enzyme immunoassay using egg yolk antibodies [J].

Blais, BW ;

Phillippe, L .

JOURNAL OF FOOD PROTECTION, 2001, 64 (06) :895-898

[4]

BLAIS BW, 1997, RRD MICROBIOL, V1, P155

[5]

BONHEVI JS, 1995, Z LEBENSM UNTERS FOR, V201, P371

[6] A SANDWICH ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR THE QUANTITATION OF SELECTED PEANUT PROTEINS IN FOODS [J].

HEFLE, SL ;

BUSH, RK ;

YUNGINGER, JW ;

CHU, FS .

JOURNAL OF FOOD PROTECTION, 1994, 57 (05) :419-423

[7] Comparison of different immunochemical methods for the detection and quantification of hazelnut proteins in food products [J].

Koppelman, SJ ;

Knulst, AC ;

Koers, WJ ;

Penninks, AH ;

Peppelman, H ;

Vlooswijk, R ;

Pigmans, I ;

van Duijn, G ;

Hessing, M .

JOURNAL OF IMMUNOLOGICAL METHODS, 1999, 229 (1-2) :107-120

[8] Immunostimulatory actions of lactobacilli:: Mitogenic induction of antibody production and spleen cell proliferation by Lactobacillus delbrueckii subsp bulgaricus and Lactobacillus acidophilus [J].

Easo, JG ;

Measham, JD ;

Munroe, J ;

Green-Johnson, JM .

FOOD AND AGRICULTURAL IMMUNOLOGY, 2002, 14 (01) :73-83

[9] Development of a rapid dipstick immunoassay for the detection of peanut contamination of food [J].

Mills, ENC ;

Potts, A ;

Plumb, GW ;

Lambert, N ;

Morgan, MRA .

FOOD AND AGRICULTURAL IMMUNOLOGY, 1997, 9 (01) :37-50

[10] BRAZIL NUT (BERTHOLLETIA-EXCELSA HBK) PROTEINS - FRACTIONATION, COMPOSITION, AND IDENTIFICATION OF A SULFUR-RICH PROTEIN [J].

SUN, SSM ;

LEUNG, FW ;

TOMIC, JC .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1987, 35 (02) :232-235

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