Batch and Continuous Extraction of Bromelain Enzyme by Reversed Micelles

被引：21

作者：

Frattini Fileti, Ana Maria ^{[1
]}

Fischer, Gilvan Anderson ^{[1
]}

Curvelo Santana, Jose Carlos ^{[1
]}

Tambourgi, Elias Basile ^{[1
]}

机构：

[1] Univ Estadual Campinas, Fac Engn Quim, BR-13083970 Campinas, SP, Brazil

来源：

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2009年 / 52卷 / 05期

关键词：

reversed micelles; liquid-liquid extraction; bromelain; pineapple; optimization; surface response; ZEA-MAYS MALT; OPTIMIZATION; MEMBRANE; RECOVERY; AMYLASES; ATPS;

D O I：

10.1590/S1516-89132009000500021

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The main aim of this study was to optimize the conditions for bromelain extraction by reversed micelles from pineapple juice (Ananas comosus). The purification was carried out in batch extraction and a micro-column with pulsed caps for continuous extraction. The cationic micellar solution was made of BDBAC as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a purification factor of 3 times at the best values of surfactant agent, co-solvent and salt concentrations, pH of the back and forward extractions were, 100 mM, 10% v/v, 1 M, 3.5 and 8, respectively. For the continuous operation, independent variables optimal point was determined: ratio between light phase flow rate and total flow, rate equal to 0.67 and 1 second for the time interval between the pulses. This optimal point led to a productivity of 1.29 mL/min and a purification factor of 4.96.

引用

页码：1225 / 1234

页数：10

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