共 33 条
Epigenetic Repression of microRNA-129-2 Leads to Overexpression of SOX4 Oncogene in Endometrial Cancer
被引:234
作者:
Huang, Yi-Wen
[1
]
Liu, Joseph C.
[1
]
Deatherage, Daniel E.
[1
]
Luo, Jingqin
[2
]
Mutch, David G.
[3
]
Goodfellow, Paul J.
[3
,4
,5
]
Miller, David S.
[6
]
Huang, Tim H-M.
[1
]
机构:
[1] Ohio State Univ, Human Canc Genet Program, Ctr Comprehens Canc, Columbus, OH 43210 USA
[2] Washington Univ, Sch Med, Div Biostat, St Louis, MO 63110 USA
[3] Washington Univ, Sch Med, Dept Obstet & Gynecol, Div Gynecol Oncol, St Louis, MO 63110 USA
[4] Washington Univ, Sch Med, Dept Surg, St Louis, MO 63110 USA
[5] Siteman Canc Ctr, St Louis, MO USA
[6] UT SW Med Ctr Dallas, Dept Obstet & Gynecol, Dallas, TX USA
关键词:
DNA HYPERMETHYLATION;
EXPRESSION;
TARGET;
GENE;
CARCINOMAS;
PREDICTION;
MECHANISM;
D O I:
10.1158/0008-5472.CAN-09-1499
中图分类号:
R73 [肿瘤学];
学科分类号:
100214 ;
摘要:
Genetic amplification, mutation, and translocation are known to play a causal role in the upregulation of an oncogene in cancer cells. Here, we report an emerging role of microRNA, the epigenetic deregulation of which may also lead to this oncogenic activation. SOX4, an oncogene belonging to the SRY-related high mobility group box family, was found to be overexpressed (P < 0.005) in endometrial tumors (n = 74) compared with uninvolved controls (n = 20). This gene is computationally predicted to be the target of a microRNA, miR-129-2. When compared with the matched endometria, the expression of miR-129-2 was lost in 27 of 31 primary endometrial tumors that also showed a concomitant gain of SOX4 expression (P < 0.001). This inverse relationship is associated with hypermethylation of the miR-129-2 CpG island, which was observed in endometrial cancer cell lines (n = 6) and 68% of 117 endometrioid endometrial tumors analyzed. Reactivation of miR129-2 in cancer cells by pharmacologic induction of histone acetylation and DNA demethylation resulted in decreased SOX4 expression. In addition, restoration of miR-129-2 by cell transfection led to decreased SOX4 expression and reduced proliferation of cancer cells. Further analysis found a significant correlation of hypermethylated miR-129-2 with micro-satellite instability and MLH1 methylation status (P < 0.001) and poor overall survival (P < 0.039) in patients. Therefore, these results imply that the aberrant expression of SOX4 is, in part, caused by epigenetic repression of miR-129-2 in endometrial cancer. Unlike the notion that promoter hypomethylation may upregulate an oncogene, we present a new paradigm in which hypermethylation-mediated silencing of a microRNA derepresses its oncogenic target in cancer cells. [Cancer Res 2009;69(23):9038-46]
引用
收藏
页码:9038 / 9046
页数:9
相关论文