A label-free electrochemical immunoassay for carcinoembryonic antigen (CEA) based on gold nanoparticles (AuNPs) and nonconductive polymer film

被引:143
作者
Tang, Hao
Chen, Jinhua [1 ]
Nie, Lihua
Kuang, Yafei
Yao, Shouzhuo
机构
[1] Hunan Univ, State Key Lab Chem Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Peoples R China
[2] Hunan Normal Univ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Peoples R China
基金
中国国家自然科学基金;
关键词
gold nanoparticles; poly-o-aminophenol film; electrochemical impedance spectroscopy; immunoassay; carcinoembryonic antigen;
D O I
10.1016/j.bios.2006.04.027
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A simple and sensitive label-free electrochemical immunoassay electrode for detection of carcinoembryonic antigen (CEA) has been developed. CEA antibody (CEAAb) was covalently attached on glutathione (GSH) monolayer-modified gold nanoparticle (AuNP) and the resulting CEAAb-AuNP bioconjugates were immobilized on Au electrode by electro-copolymerization with o-aminophenol (OAP). Electrochemical impedance spectroscopy and cyclic voltammetry studies demonstrate that the formation of CEA antibody-antigen complexes increases the electron transfer resistance of [Fe(CN)(6)](3-/4-) redox pair at the poly-OAP/CEAAb-AuNP/Au electrode. The use of CEA antibody-AuNP bioconjugates and poly-OAP film could enhance the sensitivity and anti-non specific binding of the resulting immunoassay electrode. The preliminary application of poly-OAP/CEAAb-AuNP/Au electrode for detection of CEA was also evaluated. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:1061 / 1067
页数:7
相关论文
共 35 条
  • [1] ENZYME IMMUNOSENSOR .3. AMPEROMETRIC DETERMINATION OF HUMAN CHORIONIC-GONADOTROPIN BY MEMBRANE-BOUND ANTIBODY
    AIZAWA, M
    MORIOKA, A
    SUZUKI, S
    NAGAMURA, Y
    [J]. ANALYTICAL BIOCHEMISTRY, 1979, 94 (01) : 22 - 28
  • [2] Electrochemical and quartz crystal microbalance detection of the cholera toxin employing horseradish peroxidase and GM1-functionalized liposomes
    Alfonta, L
    Willner, I
    Throckmorton, DJ
    Singh, AK
    [J]. ANALYTICAL CHEMISTRY, 2001, 73 (21) : 5287 - 5295
  • [3] Bard A. J., 1980, ELECTROCHEMICAL METH
  • [4] A MICROELECTROCHEMICAL ENZYME TRANSISTOR RESPONSIVE TO GLUCOSE
    BARTLETT, PN
    BIRKIN, PR
    [J]. ANALYTICAL CHEMISTRY, 1994, 66 (09) : 1552 - 1559
  • [5] ONLINE MEASUREMENT OF BRAIN GLUTAMATE WITH AN ENZYME/POLYMER-COATED TUBULAR ELECTRODE
    BERNERS, MOM
    BOUTELLE, MG
    FILLENZ, M
    [J]. ANALYTICAL CHEMISTRY, 1994, 66 (13) : 2017 - 2021
  • [6] New amperometric and potentiometric immunosensors for anti-human immunoglobulin G determinations
    Campanella, L
    Attioli, R
    Colapicchioni, C
    Tomassetti, M
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 1999, 55 (01) : 23 - 32
  • [7] Amperometric immunosensor for granulocyte-macrophage colony-stimulating factor using screen-printed electrodes
    Crowley, E
    O'Sullivan, C
    Guilbault, GG
    [J]. ANALYTICA CHIMICA ACTA, 1999, 389 (1-3) : 171 - 178
  • [8] Novel amperometric immunosensor for rapid separation-free immunoassay of carcinoembryonic antigen
    Dai, Z
    Yan, F
    Hua, Y
    Hu, XY
    Ju, HX
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2004, 287 (1-2) : 13 - 20
  • [9] Immunosensors: Electrochemical sensing and other engineering approaches
    Ghindilis, AL
    Atanasov, P
    Wilkins, M
    Wilkins, E
    [J]. BIOSENSORS & BIOELECTRONICS, 1998, 13 (01) : 113 - 131
  • [10] On the preparation, characterization, and enzymatic activity of fungal protease-gold colloid bioconjugates
    Gole, A
    Dash, C
    Soman, C
    Sainkar, SR
    Rao, M
    Sastry, M
    [J]. BIOCONJUGATE CHEMISTRY, 2001, 12 (05) : 684 - 690