A label-free electrochemical immunoassay for carcinoembryonic antigen (CEA) based on gold nanoparticles (AuNPs) and nonconductive polymer film

被引：143
作者：
Tang, Hao
Chen, Jinhua [1 ]
Nie, Lihua
Kuang, Yafei
Yao, Shouzhuo
机构：
[1] Hunan Univ, State Key Lab Chem Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Peoples R China
[2] Hunan Normal Univ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 06期
基金：
中国国家自然科学基金;
关键词：
gold nanoparticles; poly-o-aminophenol film; electrochemical impedance spectroscopy; immunoassay; carcinoembryonic antigen;
D O I：
10.1016/j.bios.2006.04.027
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A simple and sensitive label-free electrochemical immunoassay electrode for detection of carcinoembryonic antigen (CEA) has been developed. CEA antibody (CEAAb) was covalently attached on glutathione (GSH) monolayer-modified gold nanoparticle (AuNP) and the resulting CEAAb-AuNP bioconjugates were immobilized on Au electrode by electro-copolymerization with o-aminophenol (OAP). Electrochemical impedance spectroscopy and cyclic voltammetry studies demonstrate that the formation of CEA antibody-antigen complexes increases the electron transfer resistance of [Fe(CN)(6)](3-/4-) redox pair at the poly-OAP/CEAAb-AuNP/Au electrode. The use of CEA antibody-AuNP bioconjugates and poly-OAP film could enhance the sensitivity and anti-non specific binding of the resulting immunoassay electrode. The preliminary application of poly-OAP/CEAAb-AuNP/Au electrode for detection of CEA was also evaluated. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：1061 / 1067
页数：7
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