Biomimetically Ornamented Rapid Prototyping Fabrication of an Apatite-Collagen-Polycaprolactone Composite Construct with Nano-Micro-Macro Hierarchical Structure for Large Bone Defect Treatment

被引:68
作者
Wang, Jinbing [1 ]
Wu, Dingyu [2 ,3 ]
Zhang, Zhanzhao [2 ,3 ]
Li, Jun [1 ]
Shen, Yi [1 ]
Wang, Zhenxing [2 ,3 ]
Li, Yu [2 ,3 ]
Zhang, Zhi-Yong [2 ,3 ]
Sun, Jian [1 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Dept Oral & Maxillofacial Head & Neck Oncol,Shang, Shanghai 200011, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Shanghai Key Lab Tissue Engn,Dept Plast & Reconst, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China
[3] Natl Tissue Engn Ctr China, Shanghai 200241, Peoples R China
基金
中国国家自然科学基金;
关键词
rapid prototyping technology; biomimetically functionalization; hierarchical structure; bone graft substitute; bone defect treatment; osteoconduction; osteointegration; bone regeneration; MECHANICAL-PROPERTIES; CALCIUM-PHOSPHATE; MINERALIZED COLLAGEN; SURFACE MODIFICATION; SCAFFOLDS; HYDROXYAPATITE; DEPOSITION; MATRIX; ARCHITECTURE; POROSITY;
D O I
10.1021/acsami.5b08534
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Biomaterial-based bone graft substitute with favorable mechanical and biological properties could be used as an alternative to autograft for large defect treatment. Here, an apatite collagen polycaprolactone (Ap-Col-PCL) composite construct was developed with unique nano micro macro hierarchical architectures by combining rapid prototyping (RP) fabrication technology and a 3D functionalization strategy. Macroporous PCL framework was fabricated using RP technology, then functionalized by collagen incorporation and biomimetic deposition. Ap-Col-PCL composite construct was characterized with hierarchical architectures of a nanoscale (similar to 100 nm thickness and similar to 1 mu m length) platelike apatite coating on the microporous (126 +/- 18 mu m) collagen networks, which homogeneously filled the macroporous (similar to 4000 mu m) PCL frameworks and possessed a favorable hydrophilic property and compressive modulus (68.75 +/- 3.39 MPa) similar to that of cancellous bone. Moreover, in vitro cell culture assay and in vivo critical-sized bone defect implantation demonstrated that the Ap-Col-PCL construct could not only significantly increase the cell adhesion capability (2.0-fold) and promote faster cell proliferation but also successfully bridge the segmental long bone defect within 12 weeks with much more bone regeneration (5.2-fold), better osteointegration (7.2-fold), and a faster new bone deposition rate (2.9-fold). Our study demonstrated that biomimetically ornamented Ap-Col-PCL constructs exhibit a favorable mechanical property, more bone tissue ingrowth, and better osteointegration capability as an effective bone graft substitute for critical-sized bone defect treatment; meanwhile, it can also harness the advantages of RP technology, in particular, facilitating the customization of the shape and size of implants according to medical images during clinical application.
引用
收藏
页码:26244 / 26256
页数:13
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