Increased ovarian follicle atresia in obese Zucker rats is associated with enhanced expression of the forkhead transcription factor FOXO1

被引：26

作者：

Kajihara, Takeshi ^{[1
]}

Uchino, Satomi ^{[1
]}

Suzuki, Motoharu ^{[1
]}

Itakura, Atsuo ^{[1
]}

Brosens, Jan J. ^{[2
]}

Ishihara, Osamu ^{[1
]}

机构：

[1] Saitama Med Univ, Dept Obstet & Gynecol, Moroyama, Saitama 3500495, Japan

[2] Univ London Imperial Coll Sci Technol & Med, Inst Reprod & Dev Biol, Hammersmith Hosp, London, England

来源：

MEDICAL MOLECULAR MORPHOLOGY | 2009年 / 42卷 / 04期

关键词：

Apoptosis; Follicle; Insulin; Ovary; Granulosa cells; GRANULOSA-CELL APOPTOSIS; POLYCYSTIC-OVARY; INSULIN; FATTY; MICE; SUPPRESSION; MUTATION;

D O I：

10.1007/s00795-009-0466-7

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

It is well established that hyperinsulinemia, resulting from insulin resistance, plays a role in the pathophysiology of polycystic ovary syndrome (PCOS). The aim of this study was to investigate if ovarian follicular development and atresia are impaired in obese hyperinsulinemic (fa/fa) Zucker rats. To gain insight into the molecular mechanism of follicular atresia, we also examined the expression and localization of forkhead transcription factor FOXO1, a major regulator of cell fate decisions such as differentiation, cell-cycle arrest, and cell death. Serum insulin but not gonadotropin levels were significantly higher in obese (fa/fa) rats when compared to lean controls. Total ovarian follicle number and the percentage of atretic follicles were also significantly increased in obese (fa/fa) rats. Follicle atresia was associated with nuclear accumulation of FOXO1 transcription factor in TUNEL-positive granulosa cells. These results suggest a role for FOXO1 in granulosa cell apoptosis and increased ovarian follicle atresia associated with hyperinsulinemia.

引用

页码：216 / 221

页数：6

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