Fucoxanthin induced apoptotic cell death in oral squamous carcinoma (KB) cells

被引:10
作者
Iyappan, Petchi [1 ]
Bala, M. Devi [2 ]
Sureshkumar, M. [3 ]
Veeraraghavan, Vishnu Priya [4 ]
Palanisamy, Arulselvan [5 ]
机构
[1] Mahsa Univ, Sch Biosci, Fac Med Biosci & Nursing, Saujana Putra Campus,Jalan SP2, Jenjarom 42610, Selangor, Malaysia
[2] Muthayammal Coll Arts & Sci, Namakkal 637408, Tamil Nadu, India
[3] Muthayammal Coll Arts & Sci, Dept Zool & Biotechnol, Namakkal 637408, Tamil Nadu, India
[4] Saveetha Univ, Saveetha Inst Med & Tech Sci, Saveetha Dent Coll, Dept Biochem, Chennai 600077, Tamil Nadu, India
[5] Muthayammal Coll Arts & Sci, Muthayammal Ctr Adv Res, Namakkal 637408, Tamil Nadu, India
关键词
Oral cancer; Antioxidant; Lipid peroxidation; Cytotoxicity; Reactive oxygen species; MARINE CAROTENOID FUCOXANTHIN; CERVICAL-CANCER CELLS; DEACETYLATED PRODUCT; CYCLE ARREST; INDUCED CYTOTOXICITY; COLORIMETRIC ASSAY; OXIDATIVE STRESS; GENE-EXPRESSION; DOWN-REGULATION; UP-REGULATION;
D O I
10.6026/97320630017181
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Fucoxanthin (Fx) is an active compound commonly found in the many types of seaweed with numerous biological activities. The main goal of this investigation is to explore the effect of Fx against the cell proliferation, apoptotic induction and oxidative stress in the oral squamous (KB) cell line. Cytotoxicity of Fx was determined by MTT assay. The intracellular ROS production, mitochondrial membrane potential (MMP) and apoptosis induction in KB cells were examined through DCFH-DA, Rhodamine-123 and DAPI, and dual staining techniques. Effect of Fx on the antioxidant enzymes and lipid peroxidation in the KB cells was studied through the standard procedures. Fx treated KB cells showed morphological changes and reduced cell survival, which is exhibited by the cytotoxic activity of 50 mu M/ml (IC50) Fx against the KB cells. The Fx treatment considerably induced the apoptotosis cells (EB/AO) and decreased the MMP (Rh-123) in KB cells. Further, it was pointed out that there was an increased lipid peroxidation (LPO) with decreased antioxidants (CAT, SOD and GSH). These results concluded that Fx has the cytotoxic effect against KB cells and has the potential to induce the apoptosis via increased oxidative stress. Hence, the Fx can be a promising agent for the treatment of oral cancer and it may lead to the development of cancer therapeutics.
引用
收藏
页码:181 / 191
页数:11
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