Developmental abnormalities and neurotoxicological effects of CuO NPs on the black sea urchin Arbacia lixula by embryotoxicity assay

被引：41

作者：

Maisano, Maria ^{[1
]}

Cappello, Tiziana ^{[1
]}

Catanese, Eva ^{[1
]}

Vitale, Valeria ^{[1
]}

Natalotto, Antonino ^{[1
]}

Giannetto, Alessia ^{[1
]}

Barreca, Davide ^{[2
]}

Brunelli, Elvira ^{[3
]}

Mauceri, Angela ^{[1
]}

Fasulo, Salvatore ^{[1
]}

机构：

[1] Univ Messina, Dept Biol & Environm Sci, I-98166 Messina, Italy

[2] Univ Messina, Dept Chem Sci, I-98166 Messina, Italy

[3] Univ Calabria, Dept Biol Ecol & Earth Sci, I-87036 Cosenza, Italy

来源：

MARINE ENVIRONMENTAL RESEARCH | 2015年 / 111卷

关键词：

Sea urchin; CuO nanoparticles; Embriotoxicity test; Embryos; Larvae; Developmental abnormality; Acetylcholinesterase; Serotonin; COPPER-OXIDE NANOPARTICLES; SILVER NANOPARTICLES; TOXICITY; ACETYLCHOLINESTERASE; EMBRYOS; CELLS; ZNO; ENVIRONMENT; TEMPERATURE; METABOLISM;

D O I：

10.1016/j.marenvres.2015.05.010

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The embryotoxicity of CuO NPs was evaluated in the black sea urchin Arbacia lixula embryos, by using 24-well plates. Fertilized eggs were exposed to five doses of CuO NPs ranging from 0.07 to 20 ppb, until pluteus stage. CuO NPs suspensions in artificial seawater formed agglomerates of 80-200 nm size, and copper uptake was 2.5-fold up in larvae exposed to high NP concentrations in respect to control. Developmental delay and morphological alteration, including skeletal abnormalities, were observed, as well as impairment in cholinergic and serotonergic nervous systems. These findings suggest the potential of CuO NPs to interfere with the normal neurotransmission pathways, thus affecting larval morphogenesis. Overall, the embryotoxicity tests are effective for evaluation of nanoparticle effects on the health of aquatic biota. Furthermore, as the black sea urchin A. lixula demonstrated to be vulnerable to NP exposure, it may be a valid bioindicator in marine biomonitoring and ecotoxicological programmes. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：121 / 127

页数：7

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