Development of 3D Microvascular Networks Within Gelatin Hydrogels Using Thermoresponsive Sacrificial Microfibers

被引：84

作者：

Lee, Jung Bok ^{[1
,2
]}

Wang, Xintong ^{[1
]}

Faley, Shannon ^{[2
]}

Baer, Bradly ^{[2
]}

Balikov, Daniel A. ^{[1
]}

Sung, Hak-Joon ^{[1
]}

Bellan, Leon M. ^{[1
,2
]}

机构：

[1] Vanderbilt Univ, Dept Biomed Engn, Nashville, TN 37235 USA

[2] Vanderbilt Univ, Dept Mech Engn, Nashville, TN 37235 USA

来源：

ADVANCED HEALTHCARE MATERIALS | 2016年 / 5卷 / 07期

基金：

美国国家科学基金会;

关键词：

IN-VITRO; METHACRYLATE HYDROGELS; MICROFLUIDIC PLATFORM; CULTURED-CELLS; TISSUE; SCAFFOLDS; FABRICATION; CONSTRUCTS; MEDICINE; SYSTEM;

D O I：

10.1002/adhm.201500792

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A 3D microvascularized gelatin hydrogel is produced using thermoresponsive sacrificial poly(N-isopropylacrylamide) microfibers. The capillary-like microvascular network allows constant perfusion of media throughout the thick hydrogel, and significantly improves the viability of human neonatal dermal fibroblasts encapsulated within the gel at a high density. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：781 / 785

页数：5

共 45 条

[1]

Annabi N, 2010, TISSUE ENG PART B-RE, V16, P371, DOI 10.1089/ten.TEB.2009.0639

[2] A 3D Interconnected Microchannel Network Formed in Gelatin by Sacrificial Shellac Microfibers [J].

Bellan, Leon M. ;

Pearsall, Matthew ;

Cropek, Donald M. ;

Langer, Robert .

ADVANCED MATERIALS, 2012, 24 (38) :5187-5191

[3] Fabrication of an artificial 3-dimensional vascular network using sacrificial sugar structures [J].

Bellan, Leon M. ;

Singh, Sunil P. ;

Henderson, Peter W. ;

Porri, Teresa J. ;

Craighead, Harold G. ;

Spector, Jason A. .

SOFT MATTER, 2009, 5 (07) :1354-1357

[4] Geometric control of cell life and death [J].

Chen, CS ;

Mrksich, M ;

Huang, S ;

Whitesides, GM ;

Ingber, DE .

SCIENCE, 1997, 276 (5317) :1425-1428

[5] Fabrication of large perfusable macroporous cell-laden hydrogel scaffolds using microbial transglutaminase [J].

Chen, Pei-Yu ;

Yang, Kai-Chiang ;

Wu, Chang-Chin ;

Yu, Jeen-Huei ;

Lin, Feng-Huei ;

Sun, Jui-Sheng .

ACTA BIOMATERIALIA, 2014, 10 (02) :912-920

[6] Prevascularization of a Fibrin-Based Tissue Construct Accelerates the Formation of Functional Anastomosis with Host Vasculature [J].

Chen, Xiaofang ;

Aledia, Anna S. ;

Ghajar, Cyrus M. ;

Griffith, Craig K. ;

Putnam, Andrew J. ;

Hughes, Christopher C. W. ;

George, Steven C. .

TISSUE ENGINEERING PART A, 2009, 15 (06) :1363-1371

[7] Microfluidic scaffolds for tissue engineering [J].

Choi, Nak Won ;

Cabodi, Mario ;

Held, Brittany ;

Gleghorn, Jason P. ;

Bonassar, Lawrence J. ;

Stroock, Abraham D. .

NATURE MATERIALS, 2007, 6 (11) :908-915

[8] Formation of perfused, functional microvascular tubes in vitro [J].

Chrobak, Kenneth M. ;

Potter, Daniel R. ;

Tien, Joe .

MICROVASCULAR RESEARCH, 2006, 71 (03) :185-196

[9] Human microvasculature fabrication using thermal inkjet printing technology [J].

Cui, Xiaofeng ;

Boland, Thomas .

BIOMATERIALS, 2009, 30 (31) :6221-6227

[10]

Faley S., 2015, RSC INTERNET SERVICE

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