Localizing and Tracking Single Nanoscale Emitters in Three Dimensions with High Spatiotemporal Resolution Using a Double-Helix Point Spread Function

被引：151

作者：

Thompson, Michael A. ^{[1
]}

Lew, Matthew D. ^{[1
,2
]}

Badieirostami, Majid ^{[1
]}

Moerner, W. E. ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Elect Engn, Stanford, CA 94305 USA

来源：

NANO LETTERS | 2010年 / 10卷 / 01期

基金：

美国国家科学基金会;

关键词：

Compendex;

D O I：

10.1021/nl903295p

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Three-dimensional nanoscale localization and tracking of dim single emitters can be obtained With a widefield fluorescence microscope exhibiting a double-helix point spread function (DH-PSF), We describe in detail how the localization precision quantitatively depends upon the number of photons detected and the z position Of the nanoscale emitter, thereby showing a similar to 10 nm localization capability along x, y, and z in the limit of weak emitters, Experimental measurements are compared to Fisher information calculations of the ultimate localization precision inherent in the DH-PSF. The DH-PSF, for the first time, is used to track single quantum dots in aqueous solution and a quantum dot-labeled structure inside a living cell in three dimensions.

引用

收藏

页码：211 / 218

页数：8

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