Oligonucleotides Containing Aminated 2'-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA

被引：19

作者：

Lou, Chenguang ^{[1
]}

Samuelsen, Simone V. ^{[1
]}

Christensen, Niels Johan ^{[3
]}

Vester, Birte ^{[2
]}

Wengel, Jesper ^{[1
]}

机构：

[1] Univ Southern Denmark, Dept Phys Chem & Pharm, Biomol Nanoscale Engn Ctr, Campusvej 55, Odense M, Denmark

[2] Univ Southern Denmark, Dept Biochem & Mol Biol, Campusvej 55, Odense M, Denmark

[3] Univ Copenhagen, Dept Chem, Biomol Nanoscale Engn Ctr, Thorvaldsensvej 40, D-1871 Frederiksberg, Denmark

来源：

BIOCONJUGATE CHEMISTRY | 2017年 / 28卷 / 04期

关键词：

LOCKED NUCLEIC-ACID; TRIPLEX-FORMING OLIGONUCLEOTIDES; ENZYMATIC STABILITY; DUPLEX STABILITY; HIGH-AFFINITY; 2'-AMINO-LNA; OLIGODEOXYRIBONUCLEOTIDE; 5-METHYLCYTOSINE; THERAPEUTICS; NUCLEOSIDES;

D O I：

10.1021/acs.bioconjchem.7b00061

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mono- and diaminated 2'-amino-LNA monomers were synthesized and introduced into oligonucleotides. Each modification imparts significant stabilization of nucleic acid duplexes and triplexes, excellent sequence selectivity, and significant nuclease resistance. Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2'-amino-LNA monomers and the host oligonucleotide backbone.

引用

页码：1214 / 1220

页数：7

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