Inhibitors of polygalacturonase in calli of Orobanche aegyptiaca

被引：8

作者：

BenHod, G ^{[1
]}

Nun, NB ^{[1
]}

Tzaban, S ^{[1
]}

Mayer, AM ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,DEPT BOT,IL-91904 JERUSALEM,ISRAEL

来源：

PHYTOCHEMISTRY | 1997年 / 45卷 / 06期

关键词：

Orobanche aegyptiaca; Orobanchaceae; calli; polygalacturonase; inhibitors;

D O I：

10.1016/S0031-9422(97)00134-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of at least three distinct polygalacturonases (PGase) in callus of Orobanche was demonstrated. The PGase activity is labile and at pH 4.5 does not require activation by cations. It can be partially purified on Biogel P 100 columns and can be resolved by PAGE into several bands. Broomrape callus tissue also contains inhibitors of PGase activity. One of these is a low M-r compound, stable to boiling and removable by dialysis. An additional inhibitor precipitable by ammonium sulphate is also present. (C) 1997 Elsevier Science Ltd. All rights reserved.

引用

页码：1115 / 1121

页数：7

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