Detection of Coccidioides Antigenemia following Dissociation of Immune Complexes

被引：35

作者：

Durkin, Michelle ^{[1
,2
]}

Estok, Lois ^{[3
]}

Hospenthal, Duane ^{[4
]}

Crum-Cianflone, Nancy ^{[5
]}

Swartzentruber, Samantha ^{[1
,2
]}

Hackett, Emily ^{[1
,2
]}

Wheat, L. Joseph ^{[1
,2
]}

机构：

[1] MiraVista Diagnost, Indianapolis, IN 46241 USA

[2] MiraBella Technol, Indianapolis, IN USA

[3] El Rio Special Immunol Associates, Tucson, AZ USA

[4] Brook Army Med Ctr, Ft Sam Houston, TX USA

[5] USN, Med Ctr, San Diego, CA 92152 USA

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2009年 / 16卷 / 10期

关键词：

ENZYME-IMMUNOASSAY; DIAGNOSIS; TESTS;

D O I：

10.1128/CVI.00227-09

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Having reported that pretreatment of serum samples with EDTA at 100 C improved the sensitivity for the detection of Histoplasma antigenemia, we have evaluated this method for the detection of Coccidioides antigenemia. Urine and serum samples from patients with coccidioidomycosis were tested using the MVista Coccidioides enzyme immunoassay, and serum samples with and without EDTA-heat treatment were tested. Antigenemia was detected in 28.6% of patients whose samples were not EDTA-heat treated and in 73.1% of those whose samples were treated. Antigenuria was detected in 50% of patients. Specificity of 100% was obtained in healthy subjects, but cross-reactions were seen in 22.2% of patients with histoplasmosis or blastomycosis. EDTA-heat treatment improves the sensitivity for the detection of Coccidioides antigenemia.

引用

页码：1453 / 1456

页数：4

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