Nationwide Stepwise Emergence and Evolution of Multidrug-Resistant Campylobacter jejuni Sequence Type 5136, United Kingdom

被引：20

作者：

Lopes, Bruno S. ^{[1
]}

Strachan, Norval J. C. ^{[1
]}

Ramjee, Meenakshi ^{[1
]}

Thomson, Anne ^{[1
]}

MacRae, Marion ^{[1
]}

Shaw, Sophie ^{[1
]}

Forbes, Ken J. ^{[1
]}

机构：

[1] Univ Aberdeen, Foresterhill, Aberdeen, Scotland

来源：

EMERGING INFECTIOUS DISEASES | 2019年 / 25卷 / 07期

关键词：

ANTIMICROBIAL SUSCEPTIBILITY; MOLECULAR EPIDEMIOLOGY; ANTIBIOTIC-RESISTANCE; COMMUNITY; GENES;

D O I：

10.3201/eid2507.181572

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We examined whole-genome-sequenced Campylobacter jejuni/and C. coli from 2012-2015 isolated from birds and human stool samples in North East Scotland for the presence of antimicrobial resistance genes. We found that sequence type (ST) 5136 (clonal complex 464) was the most prevalent multidrug-resistant strain of C. jejuni exclusively associated with poultry host reservoirs and recovered from human cases of campylobacteriosis. Tetracycline resistance in ST5136 isolates was due to a tet(O/32/O) mosaic gene, ampicillin resistance was conferred by G -> T transversion in the -10 promoter region of bla(OXA-193), fluoroquinolone resistance was due to C257T change in gyrA, and aminoglycoside resistance was conferred by aac. Whole-genome analysis showed that the strain ST5136 evolved from ST464. The nationwide emergence of ST5136 was probably due to stepwise acquisition of antimicrobial resistance genes selected by high use of beta-lactam, tetracycline, fluoroquinolone, and aminoglycoside classes of drugs in the poultry industry.

引用

页码：1320 / 1329

页数：10

共 49 条

[1]

[Anonymous], SYSTEMATIC REV ASSES

[2] The RAST server: Rapid annotations using subsystems technology [J].

Aziz, Ramy K. ;

Bartels, Daniela ;

Best, Aaron A. ;

DeJongh, Matthew ;

Disz, Terrence ;

Edwards, Robert A. ;

Formsma, Kevin ;

Gerdes, Svetlana ;

Glass, Elizabeth M. ;

Kubal, Michael ;

Meyer, Folker ;

Olsen, Gary J. ;

Olson, Robert ;

Osterman, Andrei L. ;

Overbeek, Ross A. ;

McNeil, Leslie K. ;

Paarmann, Daniel ;

Paczian, Tobias ;

Parrello, Bruce ;

Pusch, Gordon D. ;

Reich, Claudia ;

Stevens, Rick ;

Vassieva, Olga ;

Vonstein, Veronika ;

Wilke, Andreas ;

Zagnitko, Olga .

BMC GENOMICS, 2008, 9 (1)

[3] Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae [J].

Benjak, Andrej ;

Avanzi, Charlotte ;

Singh, Pushpendra ;

Loiseau, Chloe ;

Girma, Selfu ;

Busso, Philippe ;

Brum Fontes, Amanda N. ;

Miyamoto, Yuji ;

Namisato, Masako ;

Bobosha, Kidist ;

Salgado, Claudio G. ;

da Silva, Moises B. ;

Bouth, Raquel C. ;

Frade, Marco A. C. ;

Bernardes Filho, Fred ;

Barreto, Josafa G. ;

Nery, Jose A. C. ;

Buehrer-Sekula, Samira ;

Lupien, Andreanne ;

Al-Samie, Abdul R. ;

Al-Qubati, Yasin ;

Alkubati, Abdul S. ;

Bretzel, Gisela ;

Vera-Cabrera, Lucio ;

Sakho, Fatoumata ;

Johnson, Christian R. ;

Kodio, Mamoudou ;

Fomba, Abdoulaye ;

Sow, Samba O. ;

Gado, Moussa ;

Konate, Ousmane ;

Stefani, Mariane M. A. ;

Penna, Gerson O. ;

Suffys, Philip N. ;

Sarno, Euzenir Nunes ;

Moraes, Milton O. ;

Rosa, Patricia S. ;

Dias Baptista, Ida M. F. ;

Spencer, John S. ;

Aseffa, Abraham ;

Matsuoka, Masanori ;

Kai, Masanori ;

Cole, Stewart T. .

NATURE COMMUNICATIONS, 2018, 9

[4]

British Poultry Council, 2016, BPC ANT STEW SCHEM L

[5] Protein length in eukaryotic and prokaryotic proteomes [J].

Brocchieri, L ;

Karlin, S .

NUCLEIC ACIDS RESEARCH, 2005, 33 (10) :3390-3400

[6] Transfer of antibiotic-resistance genes via phage-related mobile elements [J].

Brown-Jaque, Maryury ;

Calero-Caceres, William ;

Muniesa, Maite .

PLASMID, 2015, 79 :1-7

[7] Class 1 integrons and plasmid-mediated multiple resistance genes of the Campylobacter species from pediatric patient of a university hospital in Taiwan [J].

Chang, Yi-Chih ;

Tien, Ni ;

Yang, Jai-Sing ;

Lu, Chi-Cheng ;

Tsai, Fuu-Jen ;

Huang, Tsurng-Juhn ;

Wang, I-Kuan .

GUT PATHOGENS, 2017, 9

[8] A Longitudinal 6-Year Study of the Molecular Epidemiology of Clinical Campylobacter Isolates in Oxfordshire, United Kingdom [J].

Cody, Alison J. ;

McCarthy, Noel M. ;

Wimalarathna, Helen L. ;

Colles, Frances M. ;

Clark, Lorraine ;

Bowler, Ian C. J. W. ;

Maiden, Martin C. J. ;

Dingle, Kate E. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (10) :3193-3201

[9] QUINOLONE RESISTANCE IN CAMPYLOBACTER ISOLATED FROM MAN AND POULTRY FOLLOWING THE INTRODUCTION OF FLUOROQUINOLONES IN VETERINARY-MEDICINE [J].

ENDTZ, HP ;

RUIJS, GJ ;

VANKLINGEREN, B ;

JANSEN, WH ;

VANDERREYDEN, T ;

MOUTON, RP .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1991, 27 (02) :199-208

[10]

Ford G., 2015, AGR HORT DEV BOARD O

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