Increased expression of (pro)renin receptor in the remnant kidneys of 5/6 nephrectomized rats

被引：35

作者：

Hirose, Takuo ^{[1
]}

Mori, Nobuyoshi ^{[2
]}

Totsune, Kazuhito ^{[1
,3
]}

Morimoto, Ryo ^{[4
]}

Maejima, Takahiro ^{[5
]}

Kawamura, Takuya ^{[1
]}

Metoki, Hirohito ^{[1
,6
]}

Asayama, Kei ^{[3
]}

Kikuya, Masahiro ^{[1
]}

Ohkubo, Takayoshi ^{[3
,5
]}

Kohzuki, Masahiro ^{[2
]}

Takahashi, Kazuhiro ^{[7
]}

Imai, Yutaka ^{[1
,3
]}

机构：

[1] Tohoku Univ, Grad Sch Pharmaceut Sci & Med, Dept Clin Pharmacol & Therapeut, Aoba Ku, Sendai, Miyagi 9808578, Japan

[2] Tohoku Univ, Grad Sch Med, Dept Internal Med & Rehabil Sci, Aoba Ku, Sendai, Miyagi 9808574, Japan

[3] Tohoku Univ, 21st Century Ctr Excellence Program, Comprehens Res & Educ Ctr Planning Drug Dev & Cli, Aoba Ku, Sendai, Miyagi 9808578, Japan

[4] Tohoku Univ, Grad Sch Med, Div Nephrol Endocrinol & Vasc Med, Dept Med,Aoba Ku, Sendai, Miyagi 9808574, Japan

[5] Tohoku Univ, Grad Sch Pharmaceut Sci & Med, Dept Planning Drug Dev & Clin Evaluat, Aoba Ku, Sendai, Miyagi 9808575, Japan

[6] Tohoku Univ, Grad Sch Med, Dept Med Genet, Aoba Ku, Sendai, Miyagi 9808574, Japan

[7] Tohoku Univ, Grad Sch Med, Dept Endocrinol & Appl Med Sci, Aoba Ku, Sendai, Miyagi 9808575, Japan

来源：

REGULATORY PEPTIDES | 2010年 / 159卷 / 1-3期

基金：

日本学术振兴会;

关键词：

Immunohistochemistry; Organ damage; Renin-angiotensin system; RT-PCR; Western blot; RENIN-ANGIOTENSIN SYSTEM; RENIN/PRORENIN RECEPTOR; NONPROTEOLYTIC ACTIVATION; GENE-EXPRESSION; COLLECTING DUCT; BLOOD-PRESSURE; UP-REGULATION; PRORENIN; PEPTIDE; BINDING;

D O I：

10.1016/j.regpep.2009.11.006

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Recent studies have revealed that (pro)renin receptor ((P)RR), a newly identified member of the renin-angiotensin system, is associated with renal organ damage. However, there is little information regarding the regulation of (P)RR expression in various pathophysiological conditions. We therefore examined the expression of (P)RR in the remnant kidneys of rats with renal mass ablation due to 5/6 nephrectomy by quantitative RT-PCR, Western blot analysis and immunohistochemistry. Expression levels of (P)RR mRNA were significantly increased in the remnant kidneys at day 56 after nephrectomy, when compared with sham operation (about 1.6-fold, P=0.001). Western blot analysis showed that expression levels of (P)RR protein were greatly increased in the remnant kidneys at day 56, compared with sham operation (about 7.9-fold, P=0.02). The renal tubular cells were immunostained with anti-(P)RR antibody in both 5/6 nephrectomized rats and sham operated rats. The glomeruli were sporadically immunostained in 5/6 nephrectomized rats, but not in sham operated rats. These findings indicate that the intra-renal (P)RR expression is increased in the remnant kidneys of 5/6 nephrectomized rats, and suggest that (P)RR may contribute to the renal injury. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：93 / 99

页数：7

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