Cryo-EM Reveals Promoter DNA Binding and Conformational Flexibility of the General Transcription Factor TFIID

被引：25

作者：

Elmlund, Hans ^{[1
,2
]}

Baraznenok, Vera ^{[3
]}

Linder, Tomas ^{[3
]}

Szilagyi, Zsolt ^{[2
,3
]}

Rofougaran, Reza ^{[4
]}

Hofer, Anders ^{[4
]}

Hebert, Hans ^{[5
,6
]}

Lindahl, Martin ^{[5
,6
,7
]}

Gustafsson, Claes M. ^{[2
,3
]}

机构：

[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

[2] Gothenburg Univ, Dept Med Biochem & Cell Biol, SE-40530 Gothenburg, Sweden

[3] Karolinska Inst, Div Metab Dis, SE-14186 Huddinge, Sweden

[4] Umea Univ, Dept Med Biochem & Biophys, SE-90187 Umea, Sweden

[5] Karolinska Inst, Dept Biosci & Nutr, SE-14187 Huddinge, Sweden

[6] Royal Inst Technol, Sch Technol & Hlth, SE-14187 Huddinge, Sweden

[7] Lund Univ, Dept Mol Biophys, SE-22100 Lund, Sweden

来源：

STRUCTURE | 2009年 / 17卷 / 11期

基金：

瑞典研究理事会;

关键词：

RNA-POLYMERASE-II; TATA BOX; CRYSTAL-STRUCTURE; PREINITIATION COMPLEX; MINOR-GROOVE; YEAST; PROTEIN; TBP; TAFS; SUBUNIT;

D O I：

10.1016/j.str.2009.09.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-electron microscopy in combination with methods for ab initio single-particle reconstruction and heterogeneity analysis, we have produced density maps of two conformational states of Schizosaccharomyces pombe TFIID, containing and lacking TBP. We report that TBP-binding is coupled to a massive histone-fold domain rearrangement. Moreover, docking of the TBP-TAF1(N-terminus) atomic structure to the THID map and reconstruction of a TAF-promoter DNA complex helps to account for TAF-dependent regulation of promoter-TBP and promoter-TAF interactions.

引用

页码：1442 / 1452

页数：11

共 37 条

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