Dirhamnolipids secreted from Pseudomonas aeruginosa modify antifungal susceptibility of Aspergillus fumigatus by inhibiting β1,3 glucan synthase activity

被引:50
作者
Briard, Benoit [1 ,6 ]
Rasoldier, Vero [1 ]
Bomme, Perrine [2 ]
ElAouad, Noureddine [3 ,7 ]
Guerreiro, Catherine [4 ,5 ]
Chassagne, Pierre [4 ,5 ,8 ]
Muszkieta, Laetitia [1 ,9 ]
Latge, Jean-Paul [1 ]
Mulard, Laurence [4 ,5 ]
Beauvais, Anne [1 ]
机构
[1] Inst Pasteur, Unite Aspergillus, 25 Rue Docteur Roux, F-75015 Paris, France
[2] Inst Pasteur, Ultrapole, Paris, France
[3] Fdn MEDINA, Granada, Spain
[4] Inst Pasteur, Unite Chim Biomol, Paris, France
[5] Inst Pasteur, CNRS, UMR3526, Paris, France
[6] St Jude Childrens Res Hosp, 332 N Lauderdale St, Memphis, TN 38105 USA
[7] Univ Ibn Zohr, Campus Univ Ait Melloul, Agadir, Morocco
[8] Glycom AS, DTU, Lyngby, Denmark
[9] ISR, Champagne Au Mont Dor, France
关键词
CYSTIC-FIBROSIS; IN-VITRO; ANTIMICROBIAL PROPERTIES; RESPIRATORY-TRACT; AMPHOTERICIN-B; RHAMNOLIPIDS; GENE; RESISTANCE; BIOSURFACTANT; CASPOFUNGIN;
D O I
10.1038/ismej.2017.32
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Pseudomonas aeruginosa and Aspergillus fumigatus are the two microorganisms responsible for most of the chronic infections in cystic fibrosis patients. P. aeruginosa is known to produce quorumsensing controlled rhamnolipids during chronic infections. Here we show that the dirhamnolipids secreted from P. aeruginosa (i) induce A. fumigatus to produce an extracellular matrix, rich in galactosaminogalactan, 1,8-dihydroxynaphthalene (DHN)- and pyo-melanin, surrounding their hyphae, which facilitates P. aeruginosa binding and (ii) inhibit A. fumigatus growth by blocking beta 1,3 glucan synthase (GS) activity, thus altering the cell wall architecture. A. fumigatus in the presence of diRhls resulted in a growth phenotype similar to that upon its treatment with antifungal echinocandins, showing multibranched hyphae and thicker cell wall rich in chitin. The diRhl structure containing two rhamnose moieties attached to fatty acyl chain is essential for the interaction with beta 1,3 GS; however, the site of action of diRhls on GS is different from that of echinocandins, and showed synergistic antifungal effect with azoles.
引用
收藏
页码:1578 / 1591
页数:14
相关论文
共 60 条
[41]   Pseudomonas aeruginosa and their small diffusible extracellular molecules inhibit Aspergillus fumigatus biofilm formation [J].
Mowat, Eilidh ;
Rajendran, Ranjith ;
Williams, Craig ;
McCulloch, Elaine ;
Jones, Brian ;
Lang, Sue ;
Ramage, Gordon .
FEMS MICROBIOLOGY LETTERS, 2010, 313 (02) :96-102
[42]   Characteristics and consequences of airway colonization by filamentous fungi in 201 adult patients with cystic fibrosis in France [J].
Paugam, Andre ;
Baixench, Marie-Therese ;
Demazes-Dufeu, Nadine ;
Burgel, Pierre-Regis ;
Sauter, Elise ;
Kanaan, Reem ;
Dusser, Daniel ;
Dupouy-Camet, Jean ;
Hubert, Dominique .
MEDICAL MYCOLOGY, 2010, 48 :S32-S36
[43]  
Perlin DS, 2011, FUTURE MICROBIOL, V6, P441, DOI [10.2217/fmb.11.19, 10.2217/FMB.11.19]
[44]   Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo red [J].
Ram, Arthur F. J. ;
Klis, Frans M. .
NATURE PROTOCOLS, 2006, 1 (05) :2253-2256
[45]  
RIORDAN JR, 1989, SCIENCE, V245, P1066
[46]   A Polysaccharide Virulence Factor of a Human Fungal Pathogen Induces Neutrophil Apoptosis via NK Cells [J].
Robinet, Pauline ;
Baychelier, Florence ;
Fontaine, Thierry ;
Picard, Capucine ;
Debre, Patrice ;
Vieillard, Vincent ;
Latge, Jean-Paul ;
Elbim, Carole .
JOURNAL OF IMMUNOLOGY, 2014, 192 (11) :5332-5342
[47]   A Ser678Pro substitution in fks1p confers resistance to echinocandin drugs in Aspergillus fumigatus [J].
Rocha, Eleusa Maria F. ;
Garcia-Effron, Guillermo ;
Park, Steven ;
Perlin, David S. .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007, 51 (11) :4174-4176
[48]   Production of Pyomelanin, a Second Type of Melanin, via the Tyrosine Degradation Pathway in Aspergillus fumigatus [J].
Schmaler-Ripcke, Jeannette ;
Sugareva, Venelina ;
Gebhardt, Peter ;
Winkler, Robert ;
Kniemeyer, Olaf ;
Heinekamp, Thorsten ;
Brakhage, Axel A. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (02) :493-503
[49]   Influence of ferric iron on gene expression and rhamnolipid synthesis during batch cultivation of Pseudomonas aeruginosa PAO1 [J].
Schmidberger, Anke ;
Henkel, Marius ;
Hausmann, Rudolf ;
Schwartz, Thomas .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 98 (15) :6725-6737
[50]   NIH Image to ImageJ: 25 years of image analysis [J].
Schneider, Caroline A. ;
Rasband, Wayne S. ;
Eliceiri, Kevin W. .
NATURE METHODS, 2012, 9 (07) :671-675