Cryogenic X-Ray Diffraction Microscopy for Biological Samples

被引：65

作者：

Lima, Enju ^{[1
]}

Wiegart, Lutz ^{[1
]}

Pernot, Petra ^{[1
]}

Howells, Malcolm ^{[1
]}

Timmins, Joanna ^{[1
]}

Zontone, Federico ^{[1
]}

Madsen, Anders ^{[1
]}

机构：

[1] European Synchrotron Radiat Facil, F-38043 Grenoble, France

来源：

PHYSICAL REVIEW LETTERS | 2009年 / 103卷 / 19期

关键词：

PHASE RETRIEVAL; TOMOGRAPHY; RESOLUTION; SPECIMENS; RADIATION;

D O I：

10.1103/PhysRevLett.103.198102

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

X-ray diffraction microscopy (XDM) is well suited for nondestructive, high-resolution biological imaging, especially for thick samples, with the high penetration power of x rays and without limitations imposed by a lens. We developed nonvacuum, cryogenic (cryo-) XDM with hard x rays at 8 keV and report the first frozen-hydrated imaging by XDM. By preserving samples in amorphous ice, the risk of artifacts associated with dehydration or chemical fixation is avoided, ensuring the imaging condition closest to their natural state. The reconstruction shows internal structures of intact D. radiodurans bacteria in their natural contrast.

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