Quantum Dots Enhanced Ultrasensitive Detection of DNA Adducts

被引：32

作者：

Wang, Zhixin ^{[1
]}

Lu, Meiling ^{[1
]}

Wang, Xiaoli ^{[1
]}

Yin, Ruichuan ^{[1
]}

Song, Yuling ^{[1
]}

Le, X. Chris ^{[2
]}

Wang, Hailin ^{[1
]}

机构：

[1] Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China

[2] Univ Alberta, Dept Lab Med & Pathol, Edmonton, AB T6G 2G3, Canada

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 24期

基金：

中国国家自然科学基金;

关键词：

TANDEM MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; LUNG; BIOMARKERS; ASSAYS; DAMAGE; RISK;

D O I：

10.1021/ac9021105

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Here we demonstrate that quantum dots (QD) can greatly improve the ultrasensitive capillary electrophoresis-laser induced fluorescence immunoassay of trace anti-benzo(a)pyrene diol epoxide (BPDE)-DNA adducts from sensitivity to separation. We for the first time show that the target QD-antibody-DNA complex is not only effectively separated but also effectively focused by capillary electrophoresis. With the online laser-induced fluorescence detection coupled, the low limits of detection of 6.6 x 10(-21) mol in mass and 120 fM in concentration are achieved for BPDE-DNA adducts. The achieved ultrasensitivity allows for human exposure biomonitoring and shows promising applications of QD in various DNA analyses, including DNA damage.

引用

页码：10285 / 10289

页数：5

共 23 条

[1] Gene expression profiles and genetic damage in benzo(a)pyrene diol epoxide-exposed TK6 cells
Akerman, GS
Rosenzweig, BA
Domon, OE
McGarrity, LJ
Blankenship, LR
Tsai, CA
Culp, SJ
MacGregor, JT
Sistare, FD
Chen, JJ
Morris, SM
[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2004, 549 (1-2) : 43 - 64
[2] Preferential formation of benzo[a]pyrene adducts at lung cancer mutational hotspots in P53
Denissenko, MF
Pao, A
Tang, MS
Pfeifer, GP
[J]. SCIENCE, 1996, 274 (5286) : 430 - 432
[3] Ultra-performance liquid chromatography-tandem mass spectrometry for rapid and highly sensitive analysis of stereoisomers of benzo[a]pyrene diol epoxide-DNA adducts
Feng, Feng
Wang, Xiaoli
Yuan, Hancheng
Wang, Hailin
[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2009, 877 (22): : 2104 - 2112
[4] Acrolein is a major cigarette-related lung cancer agent: Preferential binding at p53 mutational hotspots and inhibition of DNA repair
Feng, Zhaohui
Hu, Wenwei
Hu, Yu
Tang, Moon-shong
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (42) : 15404 - 15409
[5] Semiconductor quantum dots for bioanalysis
Gill, Ron
Zayats, Maya
Willner, Itamar
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (40) : 7602 - 7625
[6] DNA adducts in tumour, normal peripheral lung and bronchus, and peripheral blood lymphocytes from smoking and non-smoking lung cancer patients:: correlations between tissues and detection by 32P-postlabelling and immunoassay
Gyorffy, E
Anna, L
Gyori, Z
Segesdi, J
Minárovits, J
Soltész, I
Kostic, S
Csekeo, A
Poirier, MC
Schoket, B
[J]. CARCINOGENESIS, 2004, 25 (07) : 1201 - 1209
[7] Tobacco carcinogens, their biomarkers and tobacco-induced cancer
Hecht, SS
[J]. NATURE REVIEWS CANCER, 2003, 3 (10) : 733 - 744
[8] Chemoprevention of lung carcinogenesis in addicted smokers and ex-smokers
Hecht, Stephen S.
Kassie, Fekadu
Hatsukami, Dorothy K.
[J]. NATURE REVIEWS CANCER, 2009, 9 (07) : 476 - 488
[9] Howarth M, 2008, NAT METHODS, V5, P397, DOI [10.1038/nmeth.1206, 10.1038/NMETH.1206]
[10] In vitro benzo[a]pyrene diol epoxide-induced DNA adducts and risk of squamous cell carcinoma of head and neck
Li, Donghui
Wang, Li-E
Chang, Ping
El-Naggar, Adel K.
Sturgis, Erich M.
Wei, Qingyi
[J]. CANCER RESEARCH, 2007, 67 (12) : 5628 - 5634

← 1 2 3 →