The action of adenosine deaminase (EC 3.5.4.4.) on adenosine and deoxyadenosine acetates:: The crucial role of the 5′-hydroxy group for the enzyme activity

被引：25

作者：

Ciuffreda, P ^{[1
]}

Casati, S ^{[1
]}

Santaniello, E ^{[1
]}

机构：

[1] Univ Milan, LITA Vialba, Dipartimento Sci Preclin, I-20157 Milan, Italy

来源：

TETRAHEDRON | 2000年 / 56卷 / 20期

关键词：

deamination; enzymes and enzyme reactions; nucleosides;

D O I：

10.1016/S0040-4020(00)00222-2

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

From adenosine 1,2'-deoxyadenosine 3 and 3'-deoxyadenosine 5 all the acetates were prepared by lipase-catalyzed reactions. Only the acetates with free 5'-hydroxy group were deaminated by adenosine deaminase (ADA), confirming the crucial role of 5'-OH for the enzyme activity. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：3239 / 3243

页数：5

共 22 条

[1]

ANDERSEN A, 1954, J CHEM SOC, P1882

[2]

BREDERECK H, 1940, CHEM BER, V73, P269

[3] Lipase-catalyzed protection of the hydroxy groups of the nucleosides inosine and 2′-deoxyinosine:: A new chemoenzymatic synthesis of the antiviral drug 2′,3′-dideoxyinosine [J].